Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant Cdk1-as1 with an enlarged ATP binding site | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of Cdk1-As1 mutant with cyclins 5B and 2B in reaction with different substrates, overview | Saccharomyces cerevisiae | |
0.003 | - |
Fin1 | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
0.046 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 2B | Saccharomyces cerevisiae | |
0.521 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | determination of cyclin specificity of Cdk1 during cell cycle using mutant Cdk1-as1 with an enlarged ATP binding site, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains W303 and S288C and derivatives | - |
Purification (Comment) | Organism |
---|---|
tandem purification of Cdk1 with cyclin B2 or cyclin B5 by affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ADAQHATPPKKKRKVEDPKDF | a histone peptide substrate | Saccharomyces cerevisiae | ADP + ADAQHAT(P)PPKKKRKVEDPKDF | - |
? | |
ATP + Fin1 | - |
Saccharomyces cerevisiae | ADP + phosphorylated Fin1 | - |
? | |
ATP + histone H1 | - |
Saccharomyces cerevisiae | ADP + phosphorylated histone H1 | - |
? | |
additional information | determination of cyclin specificity of Cdk1 during cell cycle using mutant Cdk1-as1 with an enlarged ATP binding site, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | determination of cyclin specificity of Cdk1 with different enzyme substrates using mutant Cdk1-as1 with an enlarged ATP binding site, overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdk1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Fin1 | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
1.9 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
3.15 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 2B | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | mutant Cdk1-as1 has an enlarged ATP binding site | Saccharomyces cerevisiae | |
cyclin B2 | i.e. Clb2 | Saccharomyces cerevisiae | |
cyclin B5 | i.e. Clb5, specificity mechanism, overview | Saccharomyces cerevisiae | |
additional information | determination of cyclin specificity of Cdk1 during cell cycle and with different enzyme substrates, overview | Saccharomyces cerevisiae |