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Literature summary for 2.7.11.22 extracted from

  • Harwell, R.M.; Mull, B.B.; Porter, D.C.; Keyomarsi, K.
    Activation of cyclin-dependent kinase 2 by full length and low molecular weight forms of cyclin E in breast cancer cells (2004), J. Biol. Chem., 279, 12695-12705.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information CDK2 is activated by phosphorylation and cyclin binding Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription and translation of CDK2 in rabbit reticulocyte lysate, expression of HA-tagged wild-type and mutant CDK2 in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
T160A site-directed mutagenesis of CDK2, phosphorylation site mutant, the mutant is activated upon binding of cyclin E Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens constitutitve activation of CDK2-cyclin E leads to G1/S deregulation and tumor progression ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein CDK2 is activated by phosphorylation at Thr160 and cyclin binding Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1
-
Homo sapiens ADP + phosphorylated histone H1
-
?
additional information constitutitve activation of CDK2-cyclin E leads to G1/S deregulation and tumor progression Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CDK2
-
Homo sapiens
cyclin-dependent kinase 2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
cyclin A competes with cyclin E isoforms Homo sapiens
cyclin E dependent on, high expression level in breast cancer cells, activating low molecular weight forms L, T1, and T2 of the cyclin E are expressed in breast cancer, modeling of CDK2 activation by cyclin E, overview Homo sapiens