Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclin A | cdk2 is dependent on, required as activating subunit, complexes with cdk2 | eukaryota |
Crystallization (Comment) | Organism |
---|---|
cdk2, X-ray diffraction structure analysis | eukaryota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | can partially substitue Mg2+ | eukaryota | |
Co2+ | can partially substitue Mg2+ | eukaryota | |
Mg2+ | dependent on, Mg2+ is the physiologic metal ion, other divalent cations are able to support nucleotide binding, but only Mn2+, Co2+, and Cd2+ can substitute Mg2+ in supporting the catalytic activity | eukaryota | |
Mn2+ | can partially substitue Mg2+ | eukaryota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | eukaryota | - |
ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
eukaryota | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | regulation by phosphorylation of the activation loop, does not affect ATP binding, but enhances the phosphotransfer rate and the substrate binding | eukaryota |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | reaction mechanism | eukaryota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
eukaryota | ADP + a phosphoprotein | - |
? | |
ATP + a protein | cdk2-cyclin A phosphorylates e.g. protein substrate p107 and peptide substrate PKTPKKAKKL, requiring a small hydrophobic patch RXL, known as a recruitment peptide | eukaryota | ADP + a phosphoprotein | - |
? | |
additional information | poor activity on free amino acids, consensus sequence of cdk2 is S/TP-XR/K | eukaryota | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme requires a cyclin as activating subunit | eukaryota |
Synonyms | Comment | Organism |
---|---|---|
CDK2 | - |
eukaryota |
cyclin-dependent kinase-2 | - |
eukaryota |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | eukaryota |