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Literature summary for 2.7.11.21 extracted from

  • Vitour, D.; Dabo, S.; Ahmadi Pour, M.; Vilasco, M.; Vidalain, P.O.; Jacob, Y.; Mezel-Lemoine, M.; Paz, S.; Arguello, M.; Lin, R.; Tangy, F.; Hiscott, J.; Meurs, E.F.
    Polo-like kinase 1 (PLK1) regulates interferon (IFN) induction by MAVS (2009), J. Biol. Chem., 284, 21797-21809.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Application

Application Comment Organism
medicine the data demonstrate the function of Plk1 as a regulator of interferon induction and provide the basis for the development of inhibitors preventing the PLK1/MAVS association to sustain innate immunity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
a full-length Myc-PLK1 plasmid is used, full-length PLK1, cloned into pDON207, is inserted into a pCINeo vector, pFLAG-PLK1-PBD and pEGFP-PLK1-PBD constructs are generated Homo sapiens

Protein Variants

Protein Variants Comment Organism
PLK1-PBD H538A/K540M PLK1 polo box domain double mutant, constructed for the determination of the specificity of the association of PLK1 with MAVS Homo sapiens
PLK1-PBD W414F/H538A/K540M PLK1 polo box domain triple mutant, constructed for the determination of the specificity of the association of PLK1 with MAVS Homo sapiens
T210D mutation in sequence of full-lenght construct Homo sapiens
T210V mutation in sequence of full-lenght construct Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Homo sapiens
-
 ADP + a phosphoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53350
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
nasopharyngeal carcinoma cell screening of a lambdagt 10 library, full-length construct Homo sapiens
-
spleen cDNA library screen, polo-box domain Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Homo sapiens ADP + a phosphoprotein
-
 ?
ATP + protein MAVS phosphorylation of the mitochondria-bound adapter protein MAVS, MAVS, mitochondrial antiviral signaling Homo sapiens ADP + phosphorylated protein MAVS
-
 ?

Synonyms

Synonyms Comment Organism
Plk1
-
 Homo sapiens
Polo-like kinase 1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function Plk1 is a regulator of interferon induction Homo sapiens