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Literature summary for 2.7.11.21 extracted from
- Regulation of IKK by phosphorylation of gamma binding domain of Ikappa Ba kinase beta by polo-like kinase 1 (2008), J. Biol. Chem., 283, 35354-35367.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| nocodazole | - |
Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the plasmids pBluescript, pBS-FLAG, pRC-beta actin and pRC-CMV are used | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K82M | kinase defective mutant | Homo sapiens |
| S137D | constitutively active mutant | Homo sapiens |
| T210D | mutant | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + protein | Homo sapiens | - |
ADP + phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P53350 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified Plk1 is obtained from HeLa cell extracts using a Q-Sepharose FF, a Heparin FF, a CM Sepharose FF, a poly(U) Sepharose 4B, and a substrate affinity column | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gammaBD of IKKbeta | Plk1 phosphorylates serines 733, 740 and 750 in the IKKgammaNEMO-binding domain, gammaBD, of IKKbeta | Homo sapiens | ADP + phosphorylated gammaBD of IKKbeta | - |
? | |
| ATP + protein | - |
Homo sapiens | ADP + phosphoprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Plk1 | - |
Homo sapiens |
| Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
in vitro kinase assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
in vitro kinase assay | Homo sapiens |
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Release 2023.1 (January 2023)
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