Application | Comment | Organism |
---|---|---|
additional information | late mitotic function of Plk1, kinase activity of Plk1 is needed for an early event in cytokinesis, acts upstream of RhoA, Plk1 activity is required for the equatorial localization of RhoA and its effectors during anaphase | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
transduction of PLK1flox/delta cells with retroviruses expressing either wild-type Plk1 or the equivalent double mutant C67V/L130G, as EGFP fusions | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C67V/L130G | localizes to centrosomes and kinetochores in mitotic cells like the wild-type, is ca. 12fold less active than wild-type in the presence of ATP, both 1-NM-PP1 and 3-MB-PP1 inhibit rapidly, selectively, and reversibly its growth in a dose-dependent manner. Acute treatment during anaphase prevents recruitment of both Plk1 itself and the Rho guanine nucleotide exchange factor Ect2 to the central spindle, abolishes RhoA GTPase localization to the equatorial cortex, and suppresses cleavage furrow formation and cell division. 3-MB-PP1 elicits a more penetrant phenotype that is virtually identical to PLK1 deletion | Homo sapiens |
additional information | homozygous deletion of PLK1 fully abrogates its function in vivo, PLK1delta/delta cells have monopolar or disorganized bipolar spindles with adjacent and immature centrosomes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 1-NM-PP1 and 3-MB-PP1 have little effect on wild-type | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Homo sapiens | 5813 | - |
kinetochore | - |
Homo sapiens | 776 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53350 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
retinal pigment epithelium | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | - |
Homo sapiens | ADP + phosphorylated casein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |