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Literature summary for 2.7.11.21 extracted from
- Polo-like kinase 1 may regulate G2/M transition of mouse fertilized eggs by means of inhibiting the phosphorylation of Tyr 15 of Cdc2 (2007), Mol. Reprod. Dev., 74, 1247-1254.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | Plk1 may regulate cell cycle progression of mouse fertilized eggs by means of inhibiting the phosphorylation of Tyr15 of Cdc2 | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the plasmids of pU6-Plk1 shRNA and pU6-Plk1 scramble shRNA are constructed | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| scytonemin | strongest inhibitory effect against Plk1 expression in mouse one-cell stage embryos, cleavage rate decreases and phosphorylation level of Tyr15 of Cdc2 increases | Mus musculus |  |
| shRNA | inhibits G2/M transition, cleavage rate decreases and phosphorylation level of Tyr15 of Cdc2 increases | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
determined by SDS-PAGE and Western Blot analysis | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + protein | Mus musculus | - |
ADP + phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Mus musculus | - |
| embryo | one-cell stage embryos, both the protein and the mRNA of Plk1 are uniformly distributed, but the kinase activity of Plk1 increases at G2/M phase and decreases at the end of M phase | Mus musculus | - |
| oocyte | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | - |
Mus musculus | ADP + phosphorylated casein | - |
? | |
| ATP + protein | - |
Mus musculus | ADP + phosphoprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Plk1 | - |
Mus musculus |
| Polo-like kinase 1 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
activity assay | Mus musculus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
- |
Mus musculus | scytonemin | |
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Release 2023.1 (January 2023)
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