Application | Comment | Organism |
---|---|---|
additional information | Plk1 is responsible for causing mitotic BubR1 upshift, and attribute a kinetochore-specific function to the hyperphosphorylated form of BubR1 in the stabilization of kinetochore-microtubule interactions | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | polo-box domain mutant in which residues His 538 and Lys 540, critical for ligand binding, are changed to alanine, do not pull down BubR1 from mitotic cell lysates | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | depletion of Plk1, which leads to loss of BubR1 hyperphosphorylation, but has no significant effects on BubR1 localization | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetochore | kinetochore localization of BubR1 and Plk1 is important for BubR1 hyperphosphorylation | Homo sapiens | 776 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BubR1 | Homo sapiens | S676 is an in vivo Plk1 phosphorylation site in BubR1 | ADP + phosphorylated BubR1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53350 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BubR1 | S676 is an in vivo Plk1 phosphorylation site in BubR1 | Homo sapiens | ADP + phosphorylated BubR1 | - |
? | |
ATP + BubR1 | Plk1-BubR1 interaction is mediated by polo-box domain binding to pT620 of BubR1. S676 is a Plk1-specific phosphorylation site, site is phosphorylated during prometaphase, but dephosphorylated at metaphase upon establishment of tension between sister chromatids. Phosphorylation is not required for spindle checkpoint function but instead is important for the stability of kinetochore-microtubule interactions, timely mitotic progression, and chromosome alignment onto the metaphase plate | Homo sapiens | ADP + phosphorylated BubR1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |