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Literature summary for 2.7.11.21 extracted from

  • Reynolds, N.; Ohkura, H.
    Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase (2003), J. Cell Sci., 116, 1377-1387.
    View publication on PubMed
Search for more literature for 2.7.11.21

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of mutant K69R in a checkpoint-defective mad2-deletion strain, two-hybrid expression of wild-type and mutant enzymes with diverse protein partners for interaction study, overview Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D181N site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1 Schizosaccharomyces pombe
D181R site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1 Schizosaccharomyces pombe
D623A/H624A/K625A site-directed mutagenesis, mutation of polo box 3, mutant strain shows reduced mitotic activity, no complementation by plo1 Schizosaccharomyces pombe
E193V site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1 Schizosaccharomyces pombe
F518A/N519A site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity Schizosaccharomyces pombe
G505A site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity Schizosaccharomyces pombe
K69R site-directed mutagenesis, mutation of the kinase domain, mutant is catalytically inactive, no complementation by plo1 Schizosaccharomyces pombe
L577A site-directed mutagenesis, mutation of polo box 2, mutant strain shows reduced mitotic activity Schizosaccharomyces pombe
additional information construction of C-terminal deletions, the deletion mutants comprise residues 1-633, 1-583, and 1-483, no complementation of the mutants by plo1 Schizosaccharomyces pombe
T197V site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1 Schizosaccharomyces pombe
W49F site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity Schizosaccharomyces pombe
Y506A/Q507A/L508A site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity, no complementation by plo1 Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information intracellular localization of wild-type and mutant enzymes, overview Schizosaccharomyces pombe
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spindle spindle pole body Schizosaccharomyces pombe 5819
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe the enzyme is involved in spindle formation and septation playing an important role in cell cycle regulation ?
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 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
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 gene plo1, diverse strains and genotypes
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in spindle formation and septation playing an important role in cell cycle regulation Schizosaccharomyces pombe ?
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 ?
additional information the enzyme interacts with diverse proteins via its poloboxes, overview Schizosaccharomyces pombe ?
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 ?

Subunits

Subunits Comment Organism
More the enzyme contains 3 different polo box motifs in the non-catalytic region that are involved in protein protein interactions in the mitotic spindle formation process Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Plo1
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 Schizosaccharomyces pombe