Cloned (Comment) | Organism |
---|---|
DNA sequence determination, transient expression of FLAG-tagged wild-type and mutant enzymes in 293T cells, co-expression witg GFP, overexpression induces wild-type protein kinase Chk2 phosphorylation, but does not increase phosphorylation of Chk2 mutant T68A | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Plk1 deletion mutants comprising residues 1-480, 1-330, or 1-408, deletion mutant 1-330 shows no kinase activity | Homo sapiens |
T82A | site-directed mutagenesis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Homo sapiens | 5813 | - |
additional information | the enzyme co-localizes with the protein kinase Chk2 | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Chk2 | Homo sapiens | - |
ADP + phosphorylated Chk2 | - |
? | |
additional information | Homo sapiens | Chk2 phosphorylation at Thr68 is enhanced by overexpression of Plk1 in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Chk2 | - |
Homo sapiens | ADP + phosphorylated Chk2 | - |
? | |
ATP + Chk2 | substrate is a protein kinase, phosphorylated by Plk1 at Ser28, Thr68, and Thr26 in vitro | Homo sapiens | ADP + phosphorylated Chk2 | - |
? | |
additional information | Chk2 phosphorylation at Thr68 is enhanced by overexpression of Plk1 in vivo | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |