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Literature summary for 2.7.11.21 extracted from

  • Tsvetkov, L.; Xu, X.; Li, J.; Stern, D.F.
    Polo-like kinase 1 and Chk2 interact and co-localize to centrosomes and the midbody (2003), J. Biol. Chem., 278, 8468-8475.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, transient expression of FLAG-tagged wild-type and mutant enzymes in 293T cells, co-expression witg GFP, overexpression induces wild-type protein kinase Chk2 phosphorylation, but does not increase phosphorylation of Chk2 mutant T68A Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of Plk1 deletion mutants comprising residues 1-480, 1-330, or 1-408, deletion mutant 1-330 shows no kinase activity Homo sapiens
T82A site-directed mutagenesis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centrosome
-
Homo sapiens 5813
-
additional information the enzyme co-localizes with the protein kinase Chk2 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Chk2 Homo sapiens
-
ADP + phosphorylated Chk2
-
?
additional information Homo sapiens Chk2 phosphorylation at Thr68 is enhanced by overexpression of Plk1 in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Chk2
-
Homo sapiens ADP + phosphorylated Chk2
-
?
ATP + Chk2 substrate is a protein kinase, phosphorylated by Plk1 at Ser28, Thr68, and Thr26 in vitro Homo sapiens ADP + phosphorylated Chk2
-
?
additional information Chk2 phosphorylation at Thr68 is enhanced by overexpression of Plk1 in vivo Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Plk1
-
Homo sapiens
Polo-like kinase 1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens