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Literature summary for 2.7.11.21 extracted from

  • Ahonen, L.J.; Kallio, M.J.; Daum, J.R.; Bolton, M.; Manke, I.A.; Yaffe, M.B.; Stukenberg, P.T.; Gorbsky, G.J.
    Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores (2005), Curr. Biol., 15, 1078-1089.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged enzyme in HeLa cells and LLCPK cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme inhibition by expression of siRNA or inhibition of enzyme expression in HeLa cells reducing the the level of 3F3/2 phosphoepitope and inhibiting normal kinetochore association, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetochore
-
Homo sapiens 776
-
kinetochore
-
Xenopus laevis 776
-
additional information localization of the enzyme is dependent on cell cycle stage Homo sapiens
-
-
additional information localization of the enzyme is dependent on cell cycle stage Xenopus laevis
-
-
spindle
-
Homo sapiens 5819
-
spindle pole
-
Homo sapiens 922
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3F3/2 kinase Homo sapiens the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity ADP + phosphorylated 3F3/2 kinase
-
?
ATP + 3F3/2 kinase Xenopus laevis the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity ADP + phosphorylated 3F3/2 kinase
-
?
additional information Xenopus laevis polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores ?
-
?
additional information Homo sapiens polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores, the enzym is required for association of Cdc20 to kinetochores ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Xenopus laevis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate consensus sequence of Plk1 is D/E-X-S/T-hydrophobic amino acid-X-D/E, X can be any amino acid, with neighbouring phosphorylation sequence L-Q-S-V-L-E being phosphorylated at the serine residue Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Xenopus laevis
-
CFPAC-1 cell
-
Homo sapiens
-
egg
-
Xenopus laevis
-
HeLa cell
-
Homo sapiens
-
LLC-PK cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3F3/2 kinase the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity Homo sapiens ADP + phosphorylated 3F3/2 kinase
-
?
ATP + 3F3/2 kinase the enzyme creates the 3F3/2 kinase phosphoepitope on mitotic kinetichores, depletion of enzyme in M phase cell extract leads to loss of 3F3/2 kinase activity Xenopus laevis ADP + phosphorylated 3F3/2 kinase
-
?
ATP + 3F3/2 kinase purified recombinant Plk1 or M phase cell extract, phosphoepitope mapping reveals that the 3F3/2 kinase phosphoepitope overlaps the enzyme consensus phosphorylation seqence, overview Homo sapiens ADP + phosphorylated 3F3/2 kinase
-
?
ATP + 3F3/2 kinase purified recombinant Plk1 or M phase cell extract, phosphoepitope mapping reveals that the 3F3/2 kinase phosphoepitope overlaps the enzyme consensus phosphorylation seqence, overview Xenopus laevis ADP + phosphorylated 3F3/2 kinase
-
?
additional information polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores Xenopus laevis ?
-
?
additional information polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores, the enzym is required for association of Cdc20 to kinetochores Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Plk1
-
Homo sapiens
Plk1
-
Xenopus laevis
Plx1
-
Xenopus laevis
Polo-like kinase 1
-
Homo sapiens
Polo-like kinase 1
-
Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Xenopus laevis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Xenopus laevis