Cloned (Comment) | Organism |
---|---|
expression as a GST (glutathione transferase)-fusion protein in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S366A | activity with RKKFGESEKTKTKEFL is decreased approximately 90% compared to wild-type activity after preincubation for 60 min with MgATP2- | Homo sapiens |
S78A | activity with RKKFGESEKTKTKEFL is decreased approximately 50% compared to wild-type activity after preincubation for 60 min with MgATP2- | Homo sapiens |
T348A | activity with RKKFGESEKTKTKEFL is decreased approximately 90% compared to wild-type activity after preincubation for 60 min with MgATP2-. The mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type | Homo sapiens |
T348D | the mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type | Homo sapiens |
T348E | the mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+/calmodulin-dependent enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation sites: Ser61, Ser66, Ser78, Ser366, Ser445, Ser474, Ser491, Thr348, Thr353. The autophosphorylation sites Thr348, Thr353, Ser366 and Ser445 are are highly conserved among vertebrate elongation factor 2 kinases. None of the sites lies in the catalytic domain. Ser366 is phosphorylated not only autocatalytically but also in trans by other kinases. Thr348 appears to be constitutively autophosphorylated in vitro. Thr348, and presumably its autophosphorylation, are critical for the ability of the enzyme to phosphorylate substrates in trans | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RKKFGESEKTKTKEFL | - |
Homo sapiens | ADP + ? | - |
? | |
ATP + [elongation factor 2] | Thr348, and presumably its autophosphorylation, are critical for the ability of the enzyme to phosphorylate substrates in trans | Homo sapiens | ADP + [elongation factor 2] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eEF2K | - |
Homo sapiens |