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Literature summary for 2.7.11.20 extracted from
- Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398 (2004), J. Biol. Chem., 279, 12220-12231.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | - |
Homo sapiens |  |
| 2-deoxy-D-glucose | - |
Rattus norvegicus | |
| 5-amino-4-imidazolecarboxyamide riboside | - |
Homo sapiens | |
| 5-amino-4-imidazolecarboxyamide riboside | - |
Rattus norvegicus | |
| Calmodulin | dependent on | Rattus norvegicus | |
| Calmodulin | completely dependent on | Homo sapiens | |
| carbonyl cyanide 3-chlorophenylhydrazone | - |
Homo sapiens | |
| additional information | ATP depletion activates the enzyme | Homo sapiens | |
| additional information | ATP depletion activates the enzyme in cardiomyocytes | Rattus norvegicus | |
| Rapamycin | - |
Homo sapiens | |
| Rapamycin | - |
Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged wild-type enzyme and of a FLAG-tagged mutant enzyme in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S398A | site-directed mutagenesis, mutation of a phosphorylation site, no phosphorylation by AMPK, altered regulation by phosphorylation compared to the wild-type enzyme | Homo sapiens |
| S78A | site-directed mutagenesis, mutation of a phosphorylation site, altered regulation by phosphorylation compared to the wild-type enzyme, but the mutation does not influence AMPK | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | completely dependent on, activates | Homo sapiens | |
| Ca2+ | dependent on, activates | Rattus norvegicus | |
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [elongation factor 2] | Rattus norvegicus | - |
ADP + [elongation factor 2]phosphate | - |
? | |
| ATP + [elongation factor 2] | Homo sapiens | enzyme regulation by de-/phosphorylation, overview | ADP + [elongation factor 2]phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs autophosphorylation, stimulation of AMP-activated protein kinase, EC 2.7.11.11, by AMP leads to activation of the enzyme and to its phosphorylation at Ser398 in the regulatory domain, other phosphorylation sites of the enzyme are Ser78, Ser359, Ser377, and Ser366 | Rattus norvegicus |
| phosphoprotein | the enzyme performs autophosphorylation, stimulation of AMP-activated protein kinase, EC 2.7.11.11, by AMP leads to activation of the wild-type and mutant enzyme and to its phosphorylation at Ser398 in the regulatory domain, other phosphorylation sites of the enzyme are Ser78, Ser359, Ser377, and Ser366, the latter is phosphorylated by kinases S6K1 and p90RSK inhibiting the enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged wild-type and FLAG-tagged mutant enzymes from Escherichia coli | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac myocyte | ventricle | Rattus norvegicus | - |
| heart ventricle | - |
Rattus norvegicus | - |
| KB cell | oral epidermoid carcinoma cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [elongation factor 2] | - |
Homo sapiens | ADP + [elongation factor 2]phosphate | - |
? | |
| ATP + [elongation factor 2] | - |
Rattus norvegicus | ADP + [elongation factor 2]phosphate | - |
? | |
| ATP + [elongation factor 2] | enzyme regulation by de-/phosphorylation, overview | Homo sapiens | ADP + [elongation factor 2]phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | recombinant GST-tagged enzyme peptide mapping | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| eEF2 kinase | - |
Homo sapiens |
| eEF2 kinase | - |
Rattus norvegicus |
| eukaryotic elongation factor 2 kinase | - |
Homo sapiens |
| eukaryotic elongation factor 2 kinase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
| 7 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| ATP | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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