Literature summary for 2.7.11.2 extracted from

Popov, K.M.; Kedishvili, N.Y.; Zhao, Y.; Shimomura, Y.; Crabb, D.W.; Harris, R.A.
Primary structure of pyruvate dehydrogenase kinase establishes a new family of eukaryotic protein kinases (1994), J. Biol. Chem., 268, 26602-26606.

Search for more literature for 2.7.11.2

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination, functional expression as soluble protein in Escherichia coli strain HMS 174 (DE3)	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
Dichloroacetate	highly specific	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	x * 36000, recombinant enzyme, SDS-PAGE	Rattus norvegicus
48000	-	x * 48000, native enzyme, SDS-PAGE	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]	Rattus norvegicus	catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1	ADP + [pyruvate dehydrogenase (lipoamide)] phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	Q63065	from heart	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli, large scale	Rattus norvegicus

Renatured (Commentary)

Renatured (Comment)	Organism
reconstitution after solubilization with 6 M urea	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]	-	Rattus norvegicus	ADP + [pyruvate dehydrogenase (lipoamide)] phosphate	-	ir
ATP + [pyruvate dehydrogenase (lipoamide)]	catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1	Rattus norvegicus	ADP + [pyruvate dehydrogenase (lipoamide)] phosphate	-	ir

Subunits

Subunits	Comment	Organism
?	x * 48000, native enzyme, SDS-PAGE	Rattus norvegicus
?	x * 36000, recombinant enzyme, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
More	PDK isozymes and the related branched-chain dehydrogenase kinase form a unique family of serine kinases	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Rattus norvegicus

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Release 2023.1 (January 2023)