Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dihydrolipoyl transacetylase | degree of interaction and mechanism differ for the 4 different isozymes | Mammalia | |
dihydrolipoyl transacetylase | degree of interaction and mechanism differ for the 4 different isozymes | Homo sapiens | |
dihydrolipoyl transacetylase | degree of interaction and mechanism differ for the 4 different isozymes | Rattus norvegicus | |
dihydrolipoyl transacetylase | activation in presence of a binding protein, referred to as dihydrolipoamide dehydrogenase-binding protein | Homo sapiens | |
dihydrolipoyl transacetylase | activation in presence of a binding protein, referred to as dihydrolipoamide dehydrogenase-binding protein | Rattus norvegicus | |
dihydrolipoyl transacetylase | activation depends on the buffer system, the isozyme and the reduction status of the lipoyl groups | Homo sapiens | |
dihydrolipoyl transacetylase | activation depends on the buffer system, the isozyme and the reduction status of the lipoyl groups | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
overexpression of isozyme PDK3 in Escherichia coli BL21(DE3) as His-tagged protein | Homo sapiens |
overexpression of isozymes PDK1, PDK2, and PDK4 in Escherichia coli BL21(DE3) as His-tagged proteins | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mammalia | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Mammalia | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | Homo sapiens | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme PDK3 | - |
Mammalia | - |
at least 4 different isozymes: PDK1, PDK2, PDK3, PDK4 | - |
Rattus norvegicus | - |
3 isozymes PDHK1, PDHK2, PDHK4 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tissue-specific expression, regulation mechanisms | Mammalia | - |
additional information | tissue-specific expression, regulation mechanisms | Homo sapiens | - |
additional information | tissue-specific expression, regulation mechanisms | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate | Homo sapiens |
additional information | - |
activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit | Mammalia | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit | Homo sapiens | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | the 3 serine phosphorylation sites of the E1 subunit are specifically and with different activity phosphorylated by the 4 isozymes, overview: site 1 is preferably utilized by PDK2, site 2 by PDK3, and site 3 is exclusively utilized by PDK1 | Homo sapiens | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | the 3 serine phosphorylation sites of the E1 subunit are specifically and with different activity phosphorylated by the 4 isozymes, overview: site 1 is preferably utilized by PDK2, site 2 by PDK3, and site 3 is exclusively utilized by PDK1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Mammalia | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Homo sapiens | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
additional information | activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate | Homo sapiens | ? | - |
? | |
additional information | activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDK | - |
Mammalia |
PDK | - |
Homo sapiens |
PDK | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0383 | - |
[Pyruvate dehydrogenase (lipoamide)] | phosphorylation site 3 of subunit E1, isozyme PDK1, pH 7.0, 30°C | Rattus norvegicus | |
0.0933 | - |
[Pyruvate dehydrogenase (lipoamide)] | phosphorylation site 2 of subunit E1, isozyme PDK3, pH 7.0, 30°C | Rattus norvegicus | |
0.108 | - |
[Pyruvate dehydrogenase (lipoamide)] | phosphorylation site 2 of subunit E1, isozyme PDK4, pH 7.0, 30°C | Rattus norvegicus | |
0.11 | - |
[Pyruvate dehydrogenase (lipoamide)] | phosphorylation site 1 of subunit E1, isozyme PDK1, pH 7.0, 30°C | Rattus norvegicus | |
0.277 | - |
[Pyruvate dehydrogenase (lipoamide)] | phosphorylation site 1 of subunit E1, isozyme PDK2, pH 7.0, 30°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
different buffer systems | Homo sapiens |
additional information | - |
different buffer systems | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Mammalia | |
ATP | dependent on | Homo sapiens | |
ATP | dependent on | Rattus norvegicus |