Cloned (Comment) | Organism |
---|---|
functional expression as maltose-binding protein fusion protein in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H121A | site-directed mutagenesis, 50% decreased trans- and autophosphorylation activity | Arabidopsis thaliana |
H121Q | site-directed mutagenesis, 50% decreased trans- and autophosphorylation activity | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, recombinant enzyme with maltose-binding protein cleaved off, SDS-PAGE | Arabidopsis thaliana |
86000 | - |
approximately, recombinant enzyme with maltose-binding protein cleaved off, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Arabidopsis thaliana | the enzyme is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SBJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli as a fusion protein with the maltose-binding protein, to near homogeneity | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | mechanism | Arabidopsis thaliana | |
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | His121 is involved in the catalytic reaction | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | substrate is kinase-depleted pyruvate dehydrogenase complex from Zea mays | Arabidopsis thaliana | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | the enzyme is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex | Arabidopsis thaliana | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
additional information | performs pH-dependent autophosphorylation on serine residues | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, recombinant enzyme with maltose-binding protein cleaved off, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
autophosphorylation | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Arabidopsis thaliana |