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Literature summary for 2.7.11.2 extracted from

  • Schuller, K.A.; Randall, D.D.
    Mechanism of pyruvate inhibition of plant pyruvate dehydrogenase kinase and synergism with ADP (1990), Arch. Biochem. Biophys., 278, 211-216.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive to ATP; inhibition only together with pyruvate, kinetics; synergism with pyruvate Pisum sativum
pyruvate dead-end inhibitor; kinetics; noncompetitive to ATP; synergism with ADP Pisum sativum
thiamine diphosphate in the presence of pyruvate Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
ATP pH 7.5, 25°C Pisum sativum
0.026
-
ATP
-
Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Pisum sativum 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Pisum sativum catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] Pisum sativum involved in regulation of mitochondrial pyruvate dehydrogenase complex ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
var. Alaskan Marvel
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf green leaf tissue Pisum sativum
-
seedling
-
Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]
-
Pisum sativum ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Pisum sativum ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] involved in regulation of mitochondrial pyruvate dehydrogenase complex Pisum sativum ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pisum sativum

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Pisum sativum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.21
-
ADP pH 7.5, 25°C Pisum sativum
0.325
-
pyruvate pH 7.5, 25°C Pisum sativum