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Literature summary for 2.7.11.19 extracted from

  • Terashima, M.; Fujita, Y.; Togashi, Y.; Sakai, K.; De Velasco, M.A.; Tomida, S.; Nishio, K.
    KIAA1199 interacts with glycogen phosphorylase kinase beta-subunit (PHKB) to promote glycogen breakdown and cancer cell survival (2014), Oncotarget, 5, 7040-7050.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
additional information KIAA1199 enhanced the phosphorylation of glycogen phosphorylase brain form Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + glycogen phosphorylase b Homo sapiens
-
 2 ADP + glycogen phosphorylase a
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q93100
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gastric cancer cell
-
 Homo sapiens
-
Hep-G2 cell
-
 Homo sapiens
-
KATO-III cell
-
 Homo sapiens
-
SNU-16 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + glycogen phosphorylase b
-
 Homo sapiens 2 ADP + glycogen phosphorylase a
-
 ?
2 ATP + glycogen phosphorylase b glycogen phosphorylase brain form, PYGB Homo sapiens 2 ADP + glycogen phosphorylase a
-
 ?

Subunits

Subunits Comment Organism
? x * 125000, about, beta-subunit Homo sapiens
More PHK is an enzyme comprised of four subunits, of which the gamma-subunit possesses enzymatic activity that is tightly regulated by the other three regulatory subunits in response to changes in intracellular Ca2+ concentrations (sensed by the delta-subunit, calmodulin) and cAMP levels that stimulate protein kinase A to phosphorylate the alpha- and beta-subunits (PHKA and PHKB). Upon phosphorylation, these two subunits, which basically inhibit the activity of the gamma-subunit, reduce their function as gamma-inhibitors, resulting in the enzymatic activation of PHK Homo sapiens

Synonyms

Synonyms Comment Organism
Glycogen phosphorylase kinase
-
 Homo sapiens
PhK
-
 Homo sapiens
PHKB
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
Calmodulin the delta subunit Homo sapiens

General Information

General Information Comment Organism
malfunction KIAA1199 protein silencing by siRNA results in a marked reduction in the phosphorylation of glycogen phosphorylase brain form, PYGB, by PHK Homo sapiens
metabolism proposed model of glycogen metabolism through the activation of PHK and PYG pathways in cancer cells with or without KIAA1199 overexpression, overview. PYG plays a central role in the mobilization of carbohydrate reserves in a wide variety of organs and tissues Homo sapiens
physiological function glycogen phosphorylase kinase (PHK) is a serine/threonine-specific protein kinase that phosphorylates a serine residue in glycogen phosphorylase (PYG), triggering the activation of this latter enzyme that catalyzes the phosphorolytic cleavage of the alpha-1,4 glycosidic linkages of glycogen, releasing glucose-1-phosphate as the reaction product. The glycogen phosphorylase kinase beta-subunit (PHKB) interacts with the C-terminal region of KIAA1199 protein, that is associated with cancer cell migration or invasion and a poor prognosis and with non-syndromic hearing loss. KIAA1199 protein also interacts with glycogen phosphorylase brain form (PYGB) under serum-free conditions. The interaction promotes glycogen breakdown and cancer cell survival. KIAA1199 binding protein pull down assay, overview Homo sapiens