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Literature summary for 2.7.11.19 extracted from
- Purification of rat liver phosphorylase kinase (1982), J. Biol. Chem., 257, 10798-10804.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Catalytic subunit of cAMP-dependent protein kinase | activation of nonactivated enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | partial | Rattus norvegicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, 0.0001-0.001 mM, inhibits above 0.001 mM | Rattus norvegicus | |
| Mg2+ | requirement | Rattus norvegicus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Rattus norvegicus | |
| Mg2+ | required for activity phosphorylation by (cAMP-dependent protein kinase) | Rattus norvegicus | |
| Mg2+ | required for activity and activation (by autophosphorylation) | Rattus norvegicus | |
| phosphate | requirement, phosphate containing enzyme | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 1300000 | - |
rat, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity from glycogen-rich pellet, from 1000 rats | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.426 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, in 20 mM triethanolamine-HCl, pH 7.5, 20% v/v glycerol, 1 mM DTT, 0.02% NaN3, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | very poor substrate | Rattus norvegicus | ? | - |
? | |
| ATP + glycogen synthase | at high concentration, from rabbit skeletal muscle | Rattus norvegicus | ADP + phosphoglycogen synthase | - |
? | |
| ATP + phosphorylase b | best substrate | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | liver (rat) | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | liver | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | rabbit skeletal muscle | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
| additional information | histone II-A is no substrate | Rattus norvegicus | ? | - |
? | |
| additional information | phosvitin is no substrate | Rattus norvegicus | ? | - |
? | |
| additional information | no substrate is histone V-S | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
influence of activation by protein kinase on pH-activity profile | Rattus norvegicus |
| 7 | - |
nonactivated enzyme | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.6 | about half-maximal activity at pH 6.2 and 7.6, nonactivated enzyme | Rattus norvegicus |
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