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Literature summary for 2.7.11.19 extracted from

  • Chan, K.F.J.; Graves, D.J.
    Rabbit skeletal muscle phosphorylase kinase. Interactions between subunits and influence of calmodulin on different complexes (1982), J. Biol. Chem., 257, 5956-5961.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

General Stability

General Stability Organism
Delta-Subunit remains tightly bound to alphagammadelta subunit complex even in the presence of 8 M urea Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
EGTA together with trifluoperazime additive effect Oryctolagus cuniculus
Trifluoperazine nonspecific inactivation, at high concentrations, together with EGTA additive effect Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of isolated delta subunit on kinetic parameters of nonactivated holoenzyme and alphagammadelta complex Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography on calmodulin-Sepharose 4B Oryctolagus cuniculus
catalytically active alphagammadelta complexes Oryctolagus cuniculus
catalytically active gammadelta complexes Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + synthetic pentadecapeptide from amino-terminal of glycogen synthase, i.e. Pro-Leu-Ser-Arg-Thr-Leu-Ser-Val-Ser-Ser-Leu-Pro-Gly-Leu-Glu Oryctolagus cuniculus ?
-
 ?
ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
More molecular interaction and subunit structure Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 50% loss of nonactivated enzyme activity within 15 min, 50% loss of alphagammadelta subunit complex activity within 7 min, 90% loss of gammadelta subunit complex activity within 5 min Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
Calmodulin requirement Oryctolagus cuniculus
Calmodulin calmodulin containing enzyme i.e. tightly bound delta subunit Oryctolagus cuniculus
Calmodulin activation of nonactivated enzyme and alphagammadelta subunit complex Oryctolagus cuniculus
Calmodulin interacts with alpha subunit Oryctolagus cuniculus