General Stability | Organism |
---|---|
Incubation of nonactivated enzyme with 100 mM ATP at 0°C dissociates the 23 S enzyme to active 7.5 S and 14 S subunits, with LiBr it produces 5 S subunits | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | not | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of catalytically active gamma subunit at pH 6.8 and 8.5 | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilization, no absolute requirement for catalytic subunit gamma2 | Oryctolagus cuniculus | |
Mg2+ | requirement | Oryctolagus cuniculus | |
Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Oryctolagus cuniculus | |
phosphate | requirement, phosphate containing enzyme | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE | Oryctolagus cuniculus |
86000 | - |
catalytically active gammagamma subunit complex, gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
catalytic subunit (i.e. gamma subunit, from holoenzyme by dissociation) | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nonactivated phosphorylase kinase | i.e. autophosphorylation and autoactivation | Oryctolagus cuniculus | ADP + activated phosphorylase kinase | - |
? | |
ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
ATP + synthetic tetradecapeptide | from amino-terminal of phosphorylase b | Oryctolagus cuniculus | ? | - |
? | |
additional information | no substrates are phosphorylase kinase gamma subunit | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9.5 | about half-maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.5 | Oryctolagus cuniculus |