Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of endogenous MLCK by two shRNA sequences: shMLCK1 is 5-GCTAGATTTGACTGCAAGATT-3' and shMLCK 2 is 5'-ACTGTCCTCTATGGCAATGAT-3' | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ML-7 | chemical inhibition of myosin light chain kinase (MLCK) slows down endocytosis | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
synaptic vesicle | - |
Rattus norvegicus | 8021 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, activates | Rattus norvegicus | |
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | Rattus norvegicus | myyosin II | ADP + [myosin light chain] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P18666 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hippocampus | hippocampal boutons | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | myyosin II | Rattus norvegicus | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | myosin II | Rattus norvegicus | ADP + [myosin light chain] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MLCK | - |
Rattus norvegicus |
Myl12b | - |
Rattus norvegicus |
myosin light chain kinase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 24 | assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | induced endocytosis is slowed by acute inhibition of MLCK and by downregulation of MLCK via RNA interference while the total amount of vesicle exocytosis and somatic Ca2+channel current does not change with MLCK down-regulation. Downregulation of MLCK prevents depolarization-induced phosphorylation of myosin light chain, an effect shared by blockers of Ca2+ channels and calmodulin. Downregulation of MLCK does not decrease the amount of exocytosis | Rattus norvegicus |
physiological function | myosin light chain kinase (MLCK) facilitates endocytosis of synaptic vesicles at hippocampal boutons through activity-dependent phosphorylation of myosin downstream of Ca2+/calmodulin. MLCK is an important activity-dependent regulator of vesicle recycling in hippocampal neurons, which are critical for learning and memory. MLCK positively regulates vesicle endocytosis at hippocampal boutons. MLCK regulates endocytosis by functioning between Ca2+/calmodulin and myosin | Rattus norvegicus |