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Literature summary for 2.7.11.18 extracted from

  • Hong, F.; Brizendine, R.K.; Carter, M.S.; Alcala, D.B.; Brown, A.E.; Chattin, A.M.; Haldeman, B.D.; Walsh, M.P.; Facemyer, K.C.; Baker, J.E.; Cremo, C.R.
    Diffusion of myosin light chain kinase on actin: A mechanism to enhance myosin phosphorylation rates in smooth muscle (2015), J. Gen. Physiol., 146, 267-280.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.18

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene MYLK, recombinant expression of wild-type and truncated enzyme, the latter comprising the N-terminal 75 residues of MLCK Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of a fragment comprising the N-terminal 75 residues of MLCK Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
actin cytoskeleton MLCK and the N-terminal 75 residues of MLCK move on actin bundles and stress fibers of smooth muscle cell cytoskeletons by a random one-dimensional diffusion mechanism, modelling, overview. MLCK motion is permitted only if acto–myosin and MLCK–myosin interactions are weak Homo sapiens 15629
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, activates Homo sapiens
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Homo sapiens smooth muscle myosin II ADP + [myosin light chain] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q15746
-
-

Source Tissue

Source Tissue Comment Organism Textmining
smooth muscle the abundance of active MLCK, which is tightly associated with the contractile apparatus, is low relative to that of smooth muscle myosin Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain] smooth muscle myosin II Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
additional information MLCK and truncated 1-75 MLCK interaction analysis with actin, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MLCK
-
 Homo sapiens
MYLK
-
 Homo sapiens
myosin light chain kinase
-
 Homo sapiens
SMM light chain kinase
-
 Homo sapiens
smooth muscle myosin light chain kinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function myosin light chain kinase is required for smooth muscle contraction and regulation of tone in most of the hollow organs of the body. MLCK and the N-terminal 75 residues of MLCK move on actin bundles and stress fibers of smooth muscle cell cytoskeletons by a random one-dimensional diffusion mechanism in a Ca2+/calmodulin- and ATP-dependent manner, modelling, overview. MLCK motion is permitted only if acto-myosin and MLCK-myosin interactions are weak. One-dimensional diffusion of MLCK along actin ensures that diffusion is not rate limiting for phosphorylation, allows MLCK to locate to areas in which myosin is not yet phosphorylated, and allows MLCK to avoid getting stuck on myosins that have already been phosphorylated. Diffusion of MLCK along actin filaments may be an important mechanism for enhancing the rate of smooth muscle myosin phosphorylation in smooth muscle. In the absence of added ATP or Ca2+/calmodulin, the myosin in the stress fibers is dephosphorylated and in a rigor state (no ATP) in which actin and myosin bind strongly Homo sapiens