Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.18 extracted from

  • Martinsen, A.; Dessy, C.; Morel, N.
    Regulation of calcium channels in smooth muscle: new insights into the role of myosin light chain kinase (2014), Channels, 8, 402-413.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.18

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene Myl12b, analysis of MLCK, L-type (CaV1.2), P/Q-type (CaV2.1), and T-type (CaV3.1) voltage-dependent Ca2+ channels and RPL32 mRNA expression by real-time quantitative-PCR Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information MLCK is downregulated by transfection with a small interfering RNA targeting MLCK (MLCK-siRNA) in rat small resistance mesenteric artery and in the rat embryonic aortic cell line A7r5. Expression of long (220 kDa) and short (130 kDa) MLCK is inhibited in MLCK-siRNA-transfected cells. Noradrenaline-induced contraction and Ca2+ signal are significantly depressed in MLCK-siRNA compared to scramble-siRNA-transfected small resistance mesenteric artery. Contraction and Ca2+ signal induced by high KCl and voltage-activated Ca2+ current are also significantly decreased in MLCK-siRNAtransfected resistance mesenteric artery Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ML-7
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, activates Rattus norvegicus
Mg2+ required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 short MLCK isozyme Rattus norvegicus
220000
-
 long MLCK isozyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Rattus norvegicus smooth muscle myosin ADP + [myosin light chain] phosphate
-
 ?
ATP + [myosin light chain] Rattus norvegicus Wistar smooth muscle myosin ADP + [myosin light chain] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus D3ZFU9
-
-
Rattus norvegicus Wistar D3ZFU9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A7r5 cell an embryonic thoracic aorta cell line Rattus norvegicus
-
artery small resistance mesenteric artery Rattus norvegicus
-
smooth muscle long (220 kDa) and short (130 kDa) MLCK isozymes Rattus norvegicus
-
vascular smooth muscle cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain] smooth muscle myosin Rattus norvegicus ADP + [myosin light chain] phosphate
-
 ?
ATP + [myosin light chain] smooth muscle myosin Rattus norvegicus Wistar ADP + [myosin light chain] phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ca2+-dependent kinase myosin light chain kinase
-
 Rattus norvegicus
MLCK
-
 Rattus norvegicus
myosin light chain kinase
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

General Information

General Information Comment Organism
malfunction MLCK depletion modifies voltage-operated Ca2+ channels. KCl- and AVP-induced Ca2+ signals and voltage-activated Ca2+ current are decreased in MLCK-depleted A7r5 cells. MLCK enzyme inhibition leads to a significant inhibition of cytosolic Ca2+ increase (64%) and contraction induced by 50 mM KCl (92%). Phenotype, detailed overview. Voltage-dependent Ca2+ channel gene expression is decreased by MLCK downregulation Rattus norvegicus
physiological function stimulation of vascular smooth muscle cells increases cytosolic Ca2+ concentration as a result of Ca2+ entry from the extracellular space through plasma membrane Ca2+ channels and/or Ca2+ release from intracellular stores via the sarcoplasmic reticulum Ca2+ channels. The increase in Ca2+/calmodulin complex activates myosin light chain kinase (MLCK) triggering contraction, which regulates vasoactivity and blood flow Rattus norvegicus