Literature summary for 2.7.11.18 extracted from

Xie, C.; Zhang, Y.; Wang, H.H.; Matsumoto, A.; Nakamura, A.; Ishikawa, R.; Yoshiyama, S.; Hayakawa, K.; Kohama, K.; Gao, Y.
Calcium regulation of non-kinase and kinase activities of recombinant myosin light-chain kinase and its mutants (2009), IUBMB Life, 61, 1092-1098.

Search for more literature for 2.7.11.18

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Gallus gallus
expressed in Escherichia coli BL21(DE3) cells	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Gallus gallus
Ca2+	activates	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q28824	-	-
Gallus gallus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
butyl-Toyopearl column chromatography and Sephacryl S-200 gel filtration	Gallus gallus
calmodulin-Sepharose column chromatography, DEAE 650M Toyopearl column chromatography, and Superose 6 gel filtration	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
gizzard	-	Gallus gallus	-
stomach	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + myosin light chain	20 kDa regulatory light chain of smooth muscle	Gallus gallus	ADP + myosin light chain phosphate	-	?
ATP + myosin light chain	20 kDa regulatory light chain of smooth muscle	Bos taurus	ADP + myosin light chain phosphate	-	?
additional information	the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities	Gallus gallus	?	-	?
additional information	the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
MLCK	-	Gallus gallus
MLCK	-	Bos taurus
myosin light-chain kinase	-	Gallus gallus
myosin light-chain kinase	-	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Gallus gallus
ATP	-	Bos taurus
Calmodulin	-	Gallus gallus
Calmodulin	-	Bos taurus

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Release 2023.1 (January 2023)