Activating Compound | Comment | Organism | Structure |
---|---|---|---|
F-actin | binding via the DFRXXL motifs in the N-terminal region of isozymes S-MLCK and L-MLCK, the latter also uses the six Ig-like modules in its N-terminal extension for binding, the first two of which are minimally required for microfilament binding, overview | Oryctolagus cuniculus |
Cloned (Comment) | Organism |
---|---|
expression of the long isozyme L-MLCK in murine NIH3T3 cells, expression of GFP-tagged wild-type and mutant isozymes in COS-7 cells and as His-tagged proteins in Escherichia coli strain BL21(DE3) | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncation mutants of long and short MLCKs | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + light chain myosin II | Oryctolagus cuniculus | regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization | ADP + phosphorylated light chain myosin II | - |
? | |
additional information | Oryctolagus cuniculus | the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant isozymes from Escherichia coli strain Bl21(DE3) by nickel affinity chromatograpyh | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
smooth muscle | long isozyme L-MLCK and short isozyme S-MLCK | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + light chain myosin II | regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization | Oryctolagus cuniculus | ADP + phosphorylated light chain myosin II | - |
? | |
ATP + light chain myosin II | regulatory myosin chain, phosphorylation at Ser18 and Thr19 on the N-terminus | Oryctolagus cuniculus | ADP + phosphorylated light chain myosin II | - |
? | |
additional information | the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview | Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MLCK | - |
Oryctolagus cuniculus |
myosin light chain kinase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus |