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Literature summary for 2.7.11.18 extracted from

  • Yang, C.X.; Chen, H.Q.; Chen, C.; Yu, W.P.; Zhang, W.C.; Peng, Y.J.; He, W.Q.; Wei, D.M.; Gao, X.; Zhu, M.S.
    Microfilament-binding properties of N-terminal extension of the isoform of smooth muscle long myosin light chain kinase (2006), Cell Res., 16, 367-376.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
F-actin binding via the DFRXXL motifs in the N-terminal region of isozymes S-MLCK and L-MLCK, the latter also uses the six Ig-like modules in its N-terminal extension for binding, the first two of which are minimally required for microfilament binding, overview Oryctolagus cuniculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of the long isozyme L-MLCK in murine NIH3T3 cells, expression of GFP-tagged wild-type and mutant isozymes in COS-7 cells and as His-tagged proteins in Escherichia coli strain BL21(DE3) Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
additional information construction of truncation mutants of long and short MLCKs Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + light chain myosin II Oryctolagus cuniculus regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization ADP + phosphorylated light chain myosin II
-
?
additional information Oryctolagus cuniculus the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant isozymes from Escherichia coli strain Bl21(DE3) by nickel affinity chromatograpyh Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
smooth muscle long isozyme L-MLCK and short isozyme S-MLCK Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + light chain myosin II regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization Oryctolagus cuniculus ADP + phosphorylated light chain myosin II
-
?
ATP + light chain myosin II regulatory myosin chain, phosphorylation at Ser18 and Thr19 on the N-terminus Oryctolagus cuniculus ADP + phosphorylated light chain myosin II
-
?
additional information the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview Oryctolagus cuniculus ?
-
?

Synonyms

Synonyms Comment Organism
MLCK
-
Oryctolagus cuniculus
myosin light chain kinase
-
Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Oryctolagus cuniculus