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Literature summary for 2.7.11.18 extracted from
- Differential roles of Rho-kinase and myosin light chain kinase in regulating shape, adhesion, and migration of HT1080 fibrosarcoma cells (2006), Biochem. Biophys. Res. Commun., 343, 602-608.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ML-7 | 40-60% inhibition in vivo | Homo sapiens |  |
| W-7 | 40-60% inhibition in vivo | Homo sapiens | |
| Y-27632 | 40-60% inhibition in vivo | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + light chain myosin II | Homo sapiens | the enzyme is important in regulation of shape, adhesion and migration of fibrosarcoma cells, overview | ADP + phosphorylated light chain myosin II | - |
? | |
| additional information | Homo sapiens | inhibition of MLCK results in spherical cells and marked impairment of adhesion and migration of fibrosarcoma cells, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HT-1080 cell | a fibrosarcoma cell line | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + light chain myosin II | the enzyme is important in regulation of shape, adhesion and migration of fibrosarcoma cells, overview | Homo sapiens | ADP + phosphorylated light chain myosin II | - |
? | |
| ATP + light chain myosin II | regulatory myosin chain, phosphorylation at Ser18 and Thr19 on the N-terminus | Homo sapiens | ADP + phosphorylated light chain myosin II | - |
? | |
| additional information | inhibition of MLCK results in spherical cells and marked impairment of adhesion and migration of fibrosarcoma cells, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MLCK | - |
Homo sapiens |
| myosin light chain kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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