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Literature summary for 2.7.11.18 extracted from

  • Wadgaonkar, R.; Linz-McGillem, L.; Zaiman, A.L.; Garcia, J.G.
    Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activity (2005), J. Cell. Biochem., 94, 351-364.
    View publication on PubMed
Search for more literature for 2.7.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expression of calmodulin-independent mutant MLCK1745, lacking the C-terminal part including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, and of MLVKATPdel mutant with a defective ATP-binding site in bovine pulmonary artery endothelial cells BPAEC as V5-epitope-tagged proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutant increases the TNFalpha response to upregulate NFkappaB activity in transfected endothelial cells, MLCKATPdel mutant enzyme with defective ATP binding site inhibits TNFalpha-induced NFkappaB-activation activity in transfected endothelial cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ML-7 specific inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Homo sapiens
-
 ADP + [myosin light chain] phosphate
-
 ?
additional information Homo sapiens the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from transfected bovine endothelial cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain]
-
 Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
ATP + [myosin light chain] activated phosphorylated enzyme Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
additional information the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
EC MLCK
-
 Homo sapiens
endothelial cell myosin light chain kinase
-
 Homo sapiens
MLCK
-
 Homo sapiens
myosin light chain kinase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
Calmodulin
-
 Homo sapiens