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Literature summary for 2.7.11.18 extracted from
- Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells (2004), FEBS Lett., 557, 121-124.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable expression of a constructed Ca2+/calmodulin-dependent fluorescent biosensor MLCK in HEK-293 cells | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Homo sapiens |  |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
x * 190000, recombinant Ca2+/calmodulin-dependent fluorescent biosensor MLCK, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [myosin II regulatory light chain] | Homo sapiens | involved in many cellular cytoskeletal functions | ADP + [myosin II regulatory light chain] phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| smooth muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quantification of enzyme activity using a Ca2+/calmodulin-dependent fluorescent biosensor MLCK in vitro or in vivo, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [myosin II regulatory light chain] | - |
Homo sapiens | ADP + [myosin II regulatory light chain] phosphate | - |
? | |
| ATP + [myosin II regulatory light chain] | involved in many cellular cytoskeletal functions | Homo sapiens | ADP + [myosin II regulatory light chain] phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 190000, recombinant Ca2+/calmodulin-dependent fluorescent biosensor MLCK, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MLCK | - |
Homo sapiens |
| myosine light chain kinase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| Calmodulin | dependent on | Homo sapiens | |
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Release 2023.1 (January 2023)
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