Any feedback?
Literature summary for 2.7.11.18 extracted from
- Purification and properties of myosin light-chain kinase from fast skeletal muscle (1977), Biochem. J., 167, 137-146.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by cAMP | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | free form, strong, not in the presence of excess Mg2+ | Oryctolagus cuniculus |  |
| EGTA | strong | Oryctolagus cuniculus | |
| Increasing ionic strength | up to 0.4 M NaCl, weak | Oryctolagus cuniculus | |
| additional information | no inhibition by 3',5'-cAMP; no inhibition by AMP | Oryctolagus cuniculus | |
| phosphate | up to 0.1 M, weak | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 0.2 | [rabbit white skeletal muscle myosin P-light chain] | 27°C, pH 7.6 | Oryctolagus cuniculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| sarcoplasm | - |
Oryctolagus cuniculus | 16528 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | effect depends on Mg2+ concentration | Oryctolagus cuniculus | |
| Mg2+ | requirement, varies with ATP-concentration, MgATP2- is the active substrate | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Oryctolagus cuniculus | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
new Zealand white | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | ITP, GTP, CTP or UTP cannot replace ATP | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | not specific for 22 kDa and 15 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrates are 15 kDa and 22 kDa light-chain or heavy-chain fractions of myosin from white skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is troponin | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for 18 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is molluscan adductor myosin | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone III-S from thymus | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + [rabbit white skeletal muscle myosin P-light chain] | - |
Oryctolagus cuniculus | ADP + [rabbit white skeletal muscle myosin P-light chain] phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 77000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 8.2 | about half-maximal activity at pH 5.7 and 8.2, with a small shoulder of 77% of maximal activity at 7-7.5 | Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
rapid inactivation below | Oryctolagus cuniculus |
| 6.3 | 8 | stable in 10% sucrose | Oryctolagus cuniculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
