Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | the binding site comprises residues 913-932 with a basic amphiphilic alpha-helix structure, maximal binding at 105 nM, three different isoforms of calmodulin, CaM1, CaM3, and CaM13, differentially regulate CaMK1, overview, the unphosphorylated CaMK1 is dependent on Ca2+ and calmodulin, while the autophosphorylated enzyme is not | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
construction of a cDNA library, CaMK1 DNA and amino acid sequence determination and analysis, genetic organization, expression of wild-type and mutant CaMK1s in Escherichia coli | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
L916R | site-directed mutagenesis, mutation in the calmodulin binding site residue abolishes the calmodulin binding | Nicotiana tabacum |
R924E/K925E/K926E/K927E | site-directed mutagenesis, mutations break the basic amphiphilic alpha-helix structure of the calmodulin binding site, the mutant is not capable of calmodulin binding | Nicotiana tabacum |
W919R | site-directed mutagenesis, mutation in the calmodulin binding site residue abolishes the calmodulin binding | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction and calmodulin binding kinetics | Nicotiana tabacum | |
0.0221 | - |
syntide-2 peptide | - |
Nicotiana tabacum | |
0.0445 | - |
histone IIIS | - |
Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the unphosphorylated CaMK1 is dependent on Ca2+ and calmodulin, while the autophosphorylated enzyme is not | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q84ZT8 | CaMK1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation, which renders the enzyme independent on Ca2+ and calmodulin | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone IIIS | - |
Nicotiana tabacum | ADP + phosphorylated histone IIIS | - |
? | |
ATP + syntide-2 | i.e. PLARTLSVAGLPGKK, synthetic peptide substrate | Nicotiana tabacum | ADP + phosphorylated syntide-2 | - |
? | |
ATP + syntide-2 peptide | syntide-2 peptide is L-prolyl-L-leucyl-L-alanyl-L-arginyl-L-threonyl-L-leucyl-L-seryl-L-valyl-L-alanylglycyl-L-leucyl-L-prolylglycyl-L-lysyl-L-lysine | Nicotiana tabacum | phosphorlyated syntide-2 peptide | - |
? | |
additional information | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | CaMK1 contains 11 subdomains of the kinase catalytic domain, lacks EF hands for Ca2+ binding, and is structurally similar to the mammalian enzyme | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
calcium/calmodulin-dependent protein kinase | - |
Nicotiana tabacum |
CaM-dependent kinase | - |
Nicotiana tabacum |
CaMK | - |
Nicotiana tabacum |
CaMK1 | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nicotiana tabacum |