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Literature summary for 2.7.11.17 extracted from

  • Chow, F.A.; Anderson, K.A.; Noeldner, P.K.; Means, A.R.
    The autonomous activity of calcium/calmodulin-dependent protein kinase IV is required for its role in transcription (2005), J. Biol. Chem., 280, 20530-20538.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on, activates the nonphosphorylated CaMKIV, residues F320, N321, D322, D323, N318, N324, N330, and N336 are involved in binding, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin Homo sapiens
ionomycin activates nonphosphorylated CaMKIV by increasing intracellular Ca2+ concentration Homo sapiens
additional information Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged CaMKIV in 293A cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
F320D/N321D site-directed mutagenesis, the mutation changes the sequence FNDD to DDDD, the mutant shows no binding of calmodulin and increased activity in nonphosphorylated status compared to the wild-type enzyme Homo sapiens
additional information mutation of residues N300, N306, N312, N318, N324, N330, N336, N341, and N347 for determination of residues involved in calmodulin binding, no binding of calmodulin and increased activity compared to the wild-type enzyme by nonphosphorylated mutants of N318, N324, N330, and N336, overview Homo sapiens
T200A site-directed mutagenesis, the mutant CaMKIV is not phosphorylated by CaMKKbeta and is thus incapable of developing autonomous activity and control gene transcription, the same effect occurs due to combination of T200A with calmodulin binding site mutations, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information CaMKIV contains an autoinhibitory domain which harbors the binding site for Ca2+ and calmodulin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics for binding of calmodulin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, activates the nonphosphorylated CaMKIV, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin Homo sapiens
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation at Thr200 through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity, deactivation by dephosphorylation through PP2A Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged CaMKIV from 293A cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004 0.022 recombinant nonphosphorylated, calmodulin-binding-residue mutants Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
calcium/calmodulin-dependent protein kinase IV
-
Homo sapiens
CaMKIV
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens