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Literature summary for 2.7.11.17 extracted from
- Neuronal Ca2+/calmodulin-dependent protein kinase II - discovery, progress in a quarter of a century, and perspective: implication for learning and memory (2005), Biol. Pharm. Bull., 28, 1342-1354.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | dependent on | Drosophila melanogaster |  |
| Calmodulin | dependent on | Rattus norvegicus | |
| additional information | CaMKII is activated by autophosphorylation at Thr286alpha and Thr287beta in the autoinhibition domain | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CaMKII isozymes and splicing variants, DNA and amino acid sequence determination and analysis, gene structure and genomic organization of isozyme beta | Rattus norvegicus |
| CaMKII, DNA and amino acid sequence determination and analysis, gene structure and genomic organization | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T286D | site-sirected mutagenesis of alpha isozyme residue, the mutant is impaired in autophosphorylation and completely in neurite extension | Rattus norvegicus |
| T286D | site-sirected mutagenesis of alpha isozyme residue, the mutant is impaired in autophosphorylation and partially in neurite extension | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | CaMKII is inactivated by autophosphorylation at Thr305 and Thr306 | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ka for calmodulin is 111 nM for isozyme alpha, and 21 nM for isozyme beta | Rattus norvegicus | |
| 0.00074 | - |
MAP2 | isozyme alpha | Rattus norvegicus | |
| 0.00081 | - |
MAP2 | isozyme beta | Rattus norvegicus | |
| 0.013 | - |
ATP | isozyme beta | Rattus norvegicus | |
| 0.014 | - |
ATP | isozyme alpha | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| additional information | subcellular distribution of isozymes alpha and beta, dendritic distribution of mRNA, overview | Rattus norvegicus | - |
- |
| synapse | - |
Drosophila melanogaster | 45202 | - |
| synapse | - |
Rattus norvegicus | 45202 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Drosophila melanogaster | |
| Ca2+ | dependent on | Rattus norvegicus | |
| Mg2+ | - |
Drosophila melanogaster | |
| Mg2+ | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
10 * 54000, alpha isozyme, + 8 * 60000, beta isozyme, CaMKII, SDS-PAGE | Rattus norvegicus |
| 60000 | - |
10 * 54000, alpha isozyme, + 8 * 60000, beta isozyme, CaMKII, SDS-PAGE | Rattus norvegicus |
| 500000 | 560000 | brain CaMKII, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MAP2 | Rattus norvegicus | - |
ADP + phosphorylated MAP2 | - |
? | |
| ATP + synapsin | Rattus norvegicus | - |
ADP + phosphorylated synapsin | - |
? | |
| additional information | Drosophila melanogaster | CaM kinase II plays a role in diverse cellular processes, overview | ? | - |
? | |
| additional information | Rattus norvegicus | CaM kinase II plays a role in diverse cellular processes, the neuronal CaM kinase II is involved in neurotransmitter synthesis and release, modulation of ion cannel activity, cellular transport, cell morphology, regulation of cytoskeleton, and neurite extension, synaptic plasticity, learning and memory, overview, CaM kinase II is involved in memory storage, functional characterization and mechanism, overview, CaMKII is involved in Ca2+-dependent regulation of tyrosine hydroxylase and tryptophan hydroxylase in catecholamin and serotonin biosynthesis, and regulation of monoamine biosynthesis in the brain, CaMKII is involved in PSD in the postsynaptic space, functional analysis including the ion channel activity regulation, e.g. of the NMDA receptor and its NR2B subunit, the glutamate receptor, and the AMPA receptor, and postsynaptic signaling, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
four CaMKII isozymes alpha, beta, gamma, delta | - |
| Rattus norvegicus | - |
four CaMKII isozymes alpha, beta, gamma, delta | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | CaMKII is activated by autophosphorylation at Thr286alpha and Thr287beta in the autoinhibition domain, CaMKII is inactivated by autophosphorylation at Thr305 and Thr306 | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from forebrain by ammonium sulfate fractionation, and calmodulin and phosphate affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | high CaM kinase II expression, highly regulated activity in brain development | Drosophila melanogaster | - |
| brain | high CaM kinase II expression, highly regulated activity in brain development, distribution overview | Rattus norvegicus | - |
| brain stem | alpha and beta isozymes | Rattus norvegicus | - |
| cerebellar Purkinje cell | isozyme alpha | Rattus norvegicus | - |
| cerebellum | alpha and beta isozymes, cerebellar nuclei | Rattus norvegicus | - |
| forebrain | alpha and beta isozymes | Rattus norvegicus | - |
| additional information | CaM kinase II is ubiquitously expressed in tissues | Drosophila melanogaster | - |
| additional information | CaM kinase II is ubiquitously expressed in tissues, especially in brain | Rattus norvegicus | - |
| neuroblastoma cell | CaMKII is induced by neural differentiation of the embryonic carcinoma cells | Rattus norvegicus | - |
| neuron | neuronal Ca2+/calmodulin-dependent protein kinase II is a major component in neurons | Drosophila melanogaster | - |
| neuron | neuronal Ca2+/calmodulin-dependent protein kinase II is a major component in neurons | Rattus norvegicus | - |
| retina | alpha and beta isozymes | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MAP2 | - |
Rattus norvegicus | ADP + phosphorylated MAP2 | - |
? | |
| ATP + synapsin | - |
Rattus norvegicus | ADP + phosphorylated synapsin | - |
? | |
| additional information | CaM kinase II plays a role in diverse cellular processes, overview | Drosophila melanogaster | ? | - |
? | |
| additional information | CaM kinase II plays a role in diverse cellular processes, the neuronal CaM kinase II is involved in neurotransmitter synthesis and release, modulation of ion cannel activity, cellular transport, cell morphology, regulation of cytoskeleton, and neurite extension, synaptic plasticity, learning and memory, overview, CaM kinase II is involved in memory storage, functional characterization and mechanism, overview, CaMKII is involved in Ca2+-dependent regulation of tyrosine hydroxylase and tryptophan hydroxylase in catecholamin and serotonin biosynthesis, and regulation of monoamine biosynthesis in the brain, CaMKII is involved in PSD in the postsynaptic space, functional analysis including the ion channel activity regulation, e.g. of the NMDA receptor and its NR2B subunit, the glutamate receptor, and the AMPA receptor, and postsynaptic signaling, overview | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | molecular conformation and structure analysis of alpha and beta isozymes | Rattus norvegicus |
| octadecamer | 10 * 54000, alpha isozyme, + 8 * 60000, beta isozyme, CaMKII, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ca2+/calmodulin-dependent protein kinase II | - |
Drosophila melanogaster |
| Ca2+/calmodulin-dependent protein kinase II | - |
Rattus norvegicus |
| CaM kinase II | - |
Drosophila melanogaster |
| CaM kinase II | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Drosophila melanogaster | |
| ATP | - |
Rattus norvegicus | |
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