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Literature summary for 2.7.11.17 extracted from

  • Ikebe, M.; Reardon, S.; Scott-Woo, G.C.; Zhou, Z.; Koda, Y.
    Purification and characterization of calmodulin-dependent multifunctional protein kinase from smooth muscle: isolation of caldesmon kinase (1990), Biochemistry, 29, 11242-11248.
    View publication on PubMed
Search for more literature for 2.7.11.17

Activating Compound

Activating Compound Comment Organism Structure
additional information after autophosphorylation the enzyme is active in the absence of Ca2+/calmodulin and even in the presence of EGTA Gallus gallus

General Stability

General Stability Organism
purified enzyme binds tightly to caldesmon, binding is abolished by high concentrations of Mg2+ Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters, association with caldesmon does not alter enzymatic properties Gallus gallus
0.0049
-
 caldesmon pH 7.5, 25°C Gallus gallus
0.012
-
 ATP pH 7.5, 25°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
myofibril tightly associated Gallus gallus 30016
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Gallus gallus
Ca2+ after autophosphorylation, the enzyme is active in the absence of Ca2+/calmodulin and even in the presence of EGTA Gallus gallus
Mg2+ activation Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + caldesmon Gallus gallus
-
 ADP + caldesmon phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate not identical but with strong binding affinity for caldesmon Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
gizzard smooth muscle Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + caldesmon
-
 Gallus gallus ADP + caldesmon phosphate
-
 ?
ATP + caldesmon less efficient than synapsin Gallus gallus ADP + caldesmon phosphate
-
 ?
ATP + casein phosphorylation at about 60% the rate of caldesmon Gallus gallus ADP + phosphocasein
-
 ?
ATP + histone calf thymus histone II-S, poor substrate Gallus gallus ADP + phosphohistone
-
 ?
ATP + histone histone III-S Gallus gallus ADP + phosphohistone
-
 ?
ATP + myosin isolated light chain of smooth-muscle myosin, phosphorylated at 80% the rate of caldesmon Gallus gallus ADP + phosphomyosin
-
 ?
ATP + synapsin brain synapsin best substrate, phosphorylated at 950% the rate of caldesmon Gallus gallus ADP + phosphosynapsin
-
 ?
additional information isozyme of calmodulin-dependent multifunctional protein kinase II in smooth-muscle Gallus gallus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
More presumably identical with EC 2.7.1.117 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
Calmodulin requirement Gallus gallus
Calmodulin after autophosphorylation, the enzyme is active in the absence of Ca2+/calmodulin and even in the presence of EGTA Gallus gallus