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Literature summary for 2.7.11.17 extracted from
- Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase (1991), Biochemistry, 30, 278-286.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-amino-6-mercaptopurine | stimulation | Rattus norvegicus |  |
| isobutylmethylxanthine | slight stimulation | Rattus norvegicus | |
| protamine | stimulation | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Aminopurine | - |
Rattus norvegicus | |
| Ca2+ | not | Rattus norvegicus | |
| Chloroquine | - |
Rattus norvegicus | |
| quercetin | at high concentrations | Rattus norvegicus | |
| staurosporine | at high concentrations | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Rattus norvegicus | |
| Mn2+ | activation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| insulin stimulated 1HIRc B-cells | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | 1HIRc B cells (overexpressing the human insulin receptor), embryonic cell line 3Y1, both with elevated kinase activity after treatment with mitogens, e.g. insulin or epidermal growth factor | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.8 | 1 | microtubule associated protein 2 as substrate | Rattus norvegicus |
| 3 | - |
myelin basic protein as substrate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + histone | 5S, 6S, 7S | Rattus norvegicus | ? | - |
? | |
| ATP + histone | 8S | Rattus norvegicus | ? | - |
? | |
| ATP + histone | not: 2AS | Rattus norvegicus | ? | - |
? | |
| ATP + microtubule-associated protein 2 | ATP preferred to GTP | Rattus norvegicus | ADP + microtubule-associated protein 2 phosphate | - |
? | |
| ATP + microtubule-associated protein 2 | phosphorylation sites are serine and threonine | Rattus norvegicus | ADP + microtubule-associated protein 2 phosphate | - |
? | |
| ATP + myelin basic protein | best substrate | Rattus norvegicus | ? | - |
? | |
| ATP + myelin basic protein | phosphorylation sites: threonine | Rattus norvegicus | ? | - |
? | |
| ATP + myosin light chain | poor | Rattus norvegicus | ? | - |
? | |
| ATP + myosin light chain | smooth-muscle, cardiac | Rattus norvegicus | ? | - |
? | |
| additional information | poor or no substrates are kinesin, myosin I, phosvitin, ATP-citrate lyase | Rattus norvegicus | ? | - |
? | |
| additional information | no substrates are protamine | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.17 | - |
myelin basic protein | - |
Rattus norvegicus |
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