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Literature summary for 2.7.11.16 extracted from

  • Vatter, P.; Stoesser, C.; Samel, I.; Gierschik, P.; Moepps, B.
    The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain (2005), FEBS J., 272, 6039-6051.
    View publication on PubMed
Search for more literature for 2.7.11.16

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in Spodoptera frugiperda Sf9 cells via baculovirus infection system, transient expression of isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D in COS-7 cells, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of isozymes mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C are membrane-associated, recombinant isoform mGRK6-D is inactive and located in the nucleus, structure-activity relationships of the enzyme C-terminus, overview Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information the variable C-terminal extension of GRK6 constitutes a domain with autoinhibitory function involving residues D560, S566, and L576 of 3 inhibitory elements and an intervening stimulatory element Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [G-protein-coupled receptor] Mus musculus
-
 ADP + [G-protein-coupled receptor]phosphate
-
 ?
additional information Mus musculus the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 4 isoforms mGRK6-A, mGRK6-B, mGRK-C, and mGRK-D by alternative splicing
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein the C-terminus of GRK6 is palmitoylated, not required for phosphorylation activity Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes mGRK6-A, mGRK6-B, and mGRK-C from Sf9 insect cells by sequential gel filtration and heparin affinity chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin light-activated rhodopsin, recombinant isoforms mGRK6-A, mGRK6-B, and mGRK-C, the latter showing highest activity Mus musculus ADP + phosphorylated rhodopsin
-
 ?
ATP + [G-protein-coupled receptor]
-
 Mus musculus ADP + [G-protein-coupled receptor]phosphate
-
 ?
additional information the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain Mus musculus ?
-
 ?
additional information structure-activity relationships of the enzyme C-terminus, overview Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
More the variable C-terminal extension of GRK6 constitutes an accessorial autoregulatory domain consisting of 3 autoregulatory elements involving residues D560, S566, and L576 and an intervening stimulatory element Mus musculus

Synonyms

Synonyms Comment Organism
G-protein-coupled receptor kinase 6
-
 Mus musculus
GRK6
-
 Mus musculus
More GRK6 belongs to the GRK4 subfamily of the GRK family Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus