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Literature summary for 2.7.11.15 extracted from

  • Meloni, A.R.; Fralish, G.B.; Kelly, P.; Salahpour, A.; Chen, J.K.; Wechsler-Reya, R.J.; Lefkowitz, R.J.; Caron, M.G.
    Smoothened signal transduction is promoted by G protein-coupled receptor kinase 2 (2006), Mol. Cell. Biol., 26, 7550-7560.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.15

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant GRK2 in C3H10T1/2 cells, transient expression in HEK-293 cells, expression of GRK2, but not catalytically inactive GRK2, synergizes with active Smoothened to mediate Gli-dependent transcription Bos taurus

Protein Variants

Protein Variants Comment Organism
K220R inactive GRK2 mutant Bos taurus
additional information knockdown of endogenous GRK2 by short hairpin RNA significantly reduces signaling in response to the Smoothened agonist SAG and also inhibits signaling induced by an oncogenic Smoothened mutant, Smo M2 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Smoothened protein Bos taurus GRK2 promotes smoothened signal transduction involved in regulation of cellular proliferation and differentiation through activation of the transcription factor Gli, overview ADP + phosphorylated Smoothened
-
 ?
additional information Bos taurus GRK2 regulates 7TM G-protein-coupled receptor activity, GRK2 promotes the association between active Smoothened and beta-arrestin 2, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Smoothened protein GRK2 promotes smoothened signal transduction involved in regulation of cellular proliferation and differentiation through activation of the transcription factor Gli, overview Bos taurus ADP + phosphorylated Smoothened
-
 ?
ATP + Smoothened protein a seven-transmembrane signaling protein Bos taurus ADP + phopshorylated Smoothened
-
 ?
additional information GRK2 regulates 7TM G-protein-coupled receptor activity, GRK2 promotes the association between active Smoothened and beta-arrestin 2, overview Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
G protein-coupled receptor kinase 2
-
 Bos taurus
GRK2
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus