Cloned (Comment) | Organism |
---|---|
co-expression of HA-tagged M1 muscarinic acetylcholine receptor and of GRK2 wild-type and mutants in CHO-K1 cells, suppression of 80% of endogenous GRK2 in HEK293 cells and in hippocampal neurons by antisense construct expression of GRK2, co-expression of wild-type and mutant GRK2s with eGFP-tagged inositol 1,4,5-trisphosphate biosensor eGFP-PHPLCdelta in HEK293 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D110A | site-directed mutagenesis, GRK2 mutant deficient in Galphaq/11 binding | Rattus norvegicus |
D110A/K220R | site-directed mutagenesis, inactive GRK2 mutant deficient in Galphaq/11 binding | Rattus norvegicus |
K220R | site-directed mutagenesis, inactive mutant GRK2 | Rattus norvegicus |
additional information | construction of GRK2-depleted hippocampal neurons and expression of a GRK2 mutant deficient in Galphaq/11 binding, overexpression of eGFP-tagged inositol 1,4,5-trisphosphate biosensor eGFP-PHPLCdelta leads to suppression of M1 muscarinic acetylcholine receptor-mediated phospholipase C signaling in hippocampal neurons through a phosphorylation-independent mechanism via binding of Galphaq/11 to the RGS homology domain of the enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + M1 muscarinic acetylcholine receptor | Rattus norvegicus | phosphorylation-dependent and -independent mechanisms in the regulation of M1 muscarinic acetylcholine receptors by G protein-coupled receptor kinase 2 in hippocampal neurons, GRK2 can inhibit the receptor-dependent signaling via phospholipase C, overview | ADP + phosphorylated M1 muscarinic acetylcholine receptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hippocampus | - |
Rattus norvegicus | - |
neuron | hippocampal | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + M1 muscarinic acetylcholine receptor | - |
Rattus norvegicus | ADP + phosphorylated M1 muscarinic acetylcholine receptor | - |
? | |
ATP + M1 muscarinic acetylcholine receptor | phosphorylation-dependent and -independent mechanisms in the regulation of M1 muscarinic acetylcholine receptors by G protein-coupled receptor kinase 2 in hippocampal neurons, GRK2 can inhibit the receptor-dependent signaling via phospholipase C, overview | Rattus norvegicus | ADP + phosphorylated M1 muscarinic acetylcholine receptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 2 | - |
Rattus norvegicus |
GRK2 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |