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Literature summary for 2.7.11.15 extracted from

  • Haske, T.N.; DeBlasi, A.; LeVine, H.
    An intact N terminus of the gamma subunit is required for the Gbetagamma stimulation of rhodopsin phosphorylation by human beta-adrenergic receptor kinase-1 but not for kinase binding (1996), J. Biol. Chem., 271, 2941-2948.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
G protein betagamma-subunit from brain, stimulates the phosphorylation of rhodopsin, but not of the peptide RRREEEEESAAA, an intact N-terminus of the gamma subunit is required for stimulation, but not for kinase binding, endoprotease Lys-C blocks stimulation, Gbetagamma binds to the C-terminal region of beta-ARK containing the pleckstrin homology domain, role of the G protein gamma-subunit Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNAs encoding the C-terminal domains Gly556-Ser670 and Pro466-Leu689 are cloned and expressed in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant beta-ARK 1 holoenzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant beta-ARK 1, expressed in Sf9 cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-adrenergic receptor
-
Homo sapiens ADP + phospho-beta-adrenergic receptor
-
?
ATP + rhodopsin in form of bovine rod outer segments Homo sapiens ADP + phosphorhodopsin
-
?
ATP + RRREEEEESAAA
-
Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens