Activating Compound | Comment | Organism | Structure |
---|---|---|---|
G protein betagamma-subunit | from brain, stimulates the phosphorylation of rhodopsin, but not of the peptide RRREEEEESAAA, an intact N-terminus of the gamma subunit is required for stimulation, but not for kinase binding, endoprotease Lys-C blocks stimulation, Gbetagamma binds to the C-terminal region of beta-ARK containing the pleckstrin homology domain, role of the G protein gamma-subunit | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNAs encoding the C-terminal domains Gly556-Ser670 and Pro466-Leu689 are cloned and expressed in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant beta-ARK 1 holoenzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant beta-ARK 1, expressed in Sf9 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | - |
Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + rhodopsin | in form of bovine rod outer segments | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + RRREEEEESAAA | - |
Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |