Activating Compound | Comment | Organism | Structure |
---|---|---|---|
G protein betagamma-subunit | activates, binding domain is localized to the C-terminal region of beta-ARK | Bos taurus | |
G protein betagamma-subunit | from brain, binds to the C-terminal half of the PH domain | Bos taurus |
Cloned (Comment) | Organism |
---|---|
wild-type beta-ARK 1 is expressed in SF9 and COS-7 cells, mutant beta-ARK 1 is cloned and expressed in COS-7 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
D635K/S636K/D637K | triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
D637K | triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
E646K | mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
K567E | mutation in the PIP2-binding region of the PH domain | Bos taurus |
K645E | mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
L647G | residue of the PH domain, mutation completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit | Bos taurus |
additional information | effects of mutations in the pleckstrin homology domain of beta-ARK on activity, Ala-insertion following Trp-643 completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit | Bos taurus |
Q642G | mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
R578N | double mutant R578N/R579N, mutation in the PIP2-binding region of the PH domain | Bos taurus |
R579N | double mutant R578N/R579N, mutation in the PIP2-binding region of the PH domain | Bos taurus |
S636K | triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain | Bos taurus |
W576A | mutation in the PIP2-binding region of the PH domain | Bos taurus |
W643A | residue of the PH domain, mutation completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme is localized to the specific membrane compartment by betagamma subunits of G proteins and phosphatidylinositol phosphates that specifically and coordinately bind to the C- and N-terminal half, respectively, of the PH domain | Bos taurus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | plays a pivotal role in phosphorylating and desensitizing G protein-coupled receptors by vitue of pleckstrin homology domain-mediated membrane translocation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
beta-ARK 1 | - |
Purification (Comment) | Organism |
---|---|
recombinant beta-ARK 1 expressed in Sf9 cells | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha2-adrenergic receptor | agonist-induced phosphorylation | Bos taurus | ADP + phospho-alpha2-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | - |
Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + muscarinic acetylcholine receptor | agonist-dependent phosphorylation, subtype 2 | Bos taurus | ADP + phospho-muscarinic acetylcholine receptor | - |
? | |
ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
additional information | mechanism and significance of the PH-domain function | Bos taurus | ? | - |
? | |
additional information | plays a pivotal role in phosphorylating and desensitizing G protein-coupled receptors by vitue of pleckstrin homology domain-mediated membrane translocation | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, rhodopsin phosphorylation by recombinant mutant beta-ARK | Bos taurus |
37 | - |
assay at, intact cell phosphorylation of muscarinic receptor subtype 2 and alpha2-adrenergic receptor by recombinant mutant beta-ARK | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |