Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isoproterenol | requirement, beta-agonist, beta-adrenergic receptor as substrate | Bos taurus | |
additional information | not effected by Ca2+ or Co2+ | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Digitonin | 0.05-0.1%, 93-95% inhibition | Bos taurus | |
additional information | not affected by Ca2+ or Co2+ | Bos taurus | |
NaCl | 20 mM, 48% inhibition, as efficient as F-, I-, NO2- or acetate, less efficient than citrate, phosphate or sulfate | Bos taurus | |
NaF | as efficient as Cl-, I-, NO2- or acetate, less efficient than citrate, phosphate or sulfate | Bos taurus | |
NaI | as efficient as Cl-, F-, NO2- or acetate, less efficient than citrate, phosphate or sulfate | Bos taurus | |
NaNO2 | as efficient as Cl-, I-, F- or acetate, less efficient than citrate, phosphate or sulfate | Bos taurus | |
Sodium acetate | as efficient as Cl-, I-, NO2- or F-, less efficient than citrate, phosphate or sulfate | Bos taurus | |
Sodium citrate | 20 mM, 97% inhibition, more efficient than phosphate, sulfate, Cl-, F-, I-, NO2- or acetate | Bos taurus | |
Sodium phosphate | 20 mM, 76% inhibition, less efficient than citrate, as good as sulfate, more efficient than Cl-, F-, I-, NO2- or acetate | Bos taurus | |
Sodium sulfate | less efficient than citrate, as good as phosphate, more efficient than Cl-, F-, I-, NO2- or acetate | Bos taurus | |
Zn2+ | 0.1-0.2 mM ZnCl2, 94-98% inhibition | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
beta-adrenergic receptor | pH 7.5, 30°C | Bos taurus | |
0.006 | - |
rhodopsin | pH 7.5, 30°C | Bos taurus | |
0.033 | - |
ATP | pH 7.5, 30°C, beta-adrenergic receptor as substrate | Bos taurus | |
0.037 | - |
ATP | pH 7.5, 30°C, rhodopsin as substrate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
plasma membrane | beta-AR may serve as membrane anchor for enzyme, translocation of beta-ARK from the cytosol to the plasma membrane | Bos taurus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus | |
Mg2+ | optimal concentration: 2-6 mM | Bos taurus | |
Mn2+ | requirement, can replace Mg2+ with 50% efficiency, optimal concentration: 1-3 mM | Bos taurus | |
additional information | not activated by Ca2+, Co2+ or Zn2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme appears to autophosphorylate | Bos taurus |
Purification (Comment) | Organism |
---|---|
20300fold, to near homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | cerebral cortex | Bos taurus | - |
brain | highest beta-ARK activity in cerebral cortex | Bos taurus | - |
cerebral cortex | highest beta-ARK activity | Bos taurus | - |
additional information | tissue distribution | Bos taurus | - |
additional information | ubiquitous enzyme | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00873 | - |
pH 7.5, 30°C | Bos taurus |
Storage Stability | Organism |
---|---|
4°C, crude enzyme: several months, stable, purified enzyme: t1/2 of 5-10 days | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | incorporation of about 9 mol phosphate/mol receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | beta-AR from hamster lung | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | beta-AR is a much better substrate than rhodopsin | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + rhodopsin | beta-AR is a much better substrate than rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | light-dependent, actual substrate: light-bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | incorporation of 0.15 mol phosphate/mol rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
GTP + beta-adrenergic receptor | agonist-occupied form of beta-AR from hamster lung, GTP can substitute ATP, 2% as effective as ATP | Bos taurus | GDP + phospho-beta-adrenergic receptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Bos taurus |
7.5 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Bos taurus |