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Literature summary for 2.7.11.15 extracted from

  • Onorato, J.J.; Palczewski, K.; Regan, J.W.; Caron, M.G.; Lefkowitz, R.J.; Benovic, J.L.
    Role of acidic amino acids in peptide substrates of the beta-adrenergic receptor kinase and rhodopsin kinase (1991), Biochemistry, 30, 5118-5125.
    View publication on PubMed
Search for more literature for 2.7.11.15

Activating Compound

Activating Compound Comment Organism Structure
isoproterenol requirement, beta-agonist, beta-adrenergic receptor as substrate Bos taurus

General Stability

General Stability Organism
Triton X-100 stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Bos taurus
peptide synthetic peptides from a variety of intracellular regions of beta2-AR specifically inhibit phosphorylation of the intact receptor, but not of peptide substrates Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9 1.3 peptide acid-rich synthetic peptides Bos taurus
1.5 4.8 peptide synthetic peptides containing a single Glu-residue Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 stabilizes Bos taurus
Triton X-100 0.02% Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
ATP + [beta-adrenergic receptor] = ADP + phospho-[beta-adrenergic receptor] mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
brain cerebral cortex Bos taurus
-
cerebral cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha2-adrenergic receptor from human platelets Bos taurus ADP + phospho-alpha2-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor phosphorylation sites Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor incorporation of 6-8 mol phosphate/mol receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
ATP + LEESSSSDHAERPPG
-
 Bos taurus ?
-
 ?
ATP + muscarinic cholinergic receptor from chick heart Bos taurus ADP + phospho-muscarinic cholinergic receptor
-
 ?
ATP + peptide e.g. Leu-Glu-Glu-Ser-Ser-Ser-Ser-Asp-His-Ala-Glu-Arg-Pro-Pro-Gly or Arg-Arg-Arg-Glu-Glu-Glu-Glu-Glu-Ser-Ala-Ala-Ala, role of acidic amino acids in peptide substrates, preference for negatively charged amino acids localized to the N-terminal side of a Ser- or Thr-residue, Ser-containing peptides are 4fold better than Thr-containing Bos taurus ADP + phosphopeptide
-
 ?
ATP + peptide synthetic Bos taurus ADP + phosphopeptide
-
 ?
ATP + rhodopsin metarhodopsin II, poor substrate Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + RRREEEEESAAA
-
 Bos taurus ?
-
 ?
additional information phosphorylates G protein coupled receptors in an agonist-dependent manner Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus