Literature summary for 2.7.11.14 extracted from

Tesmer, J.J.; Nance, M.R.; Singh, P.; Lee, H.
Structure of a monomeric variant of rhodopsin kinase at 2.5 A resolution (2012), Acta Crystallogr. Sect. F, 68, 622-625.

Search for more literature for 2.7.11.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of GRK1-L166K is determined in complex with Mg2+-ATP to 2.5 A resolution. GRK1-L166K crystallizes in a novel space group as a monomer and exhibits little overall conformational difference from wild-type GRK1	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
L116K	L166K mutation disrupts dimer interface. The structure of GRK1-L166K is determined in complex with Mg2+-ATP to 2.5 A resolution. GRK1-L166K crystallizes in a novel space group as a monomer and exhibits little overall conformational difference from wild-type GRK1	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P28327	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Bos taurus

Subunits

Subunits	Comment	Organism
dimer	wild-type, crystal structure	Bos taurus
monomer	mutant L166K, crystal structure	Bos taurus

Synonyms

Synonyms	Comment	Organism
G protein-coupled receptor kinase 1	-	Bos taurus
GRK1	-	Bos taurus
rhodopsin kinase	-	Bos taurus

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Release 2023.1 (January 2023)