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Literature summary for 2.7.11.14 extracted from

  • Chen, C.K.; Inglese, J.; Lefkowitz, R.J.; Hurley, J.B.
    Ca2+-dependent interaction of recoverin with rhodopsin kinase (1995), J. Biol. Chem., 270, 18060-18066.
    View publication on PubMed
Search for more literature for 2.7.11.14

Activating Compound

Activating Compound Comment Organism Structure
rhodopsin photolyzed rhodopsin stimulates Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
RK is cloned and expressed in SF9 cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
recoverin Ca2+-dependent inhibition; highly specific direct Ca2+-dependent interaction with RK, N-terminal myristoyl residue of recoverin enhances RK inhibition and introduces cooperativity to the inhibitory effect, quenches high-gain phosphorylation of rhodopsin in the presence of Ca2+ Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus
additional information Ca2+-independent phosphorylation of rhodopsin Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Bos taurus
66000
-
 x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rhodopsin Bos taurus specific and Ca2+-dependent recoverin/RK interaction may play an important role in photoreceptor light adaptation ADP + phosphorhodopsin
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme is autophosphorylated Bos taurus
side-chain modification purified retinal RK is farnesylated Bos taurus

Purification (Commentary)

Purification (Comment) Organism
native and recombinant RK, expressed in SF9 cells, recovering affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
eye retina Bos taurus
-
retina rod cell outer segment Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin recombinant RK expressed in SF9 cells catalyzes high-gain phosphorylation in which photoactivation of one rhodopsin molecule causes incorporation of up to several hundred phosphates into the total rhodopsin pool Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin light-dependent phosphorylation Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
ATP + rhodopsin specific and Ca2+-dependent recoverin/RK interaction may play an important role in photoreceptor light adaptation Bos taurus ADP + phosphorhodopsin
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Bos taurus
? x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus