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Literature summary for 2.7.11.14 extracted from
- A novel rhodopsin kinase in Octopus photoreceptor possesses a pleckstrin homology domain and is activated by G protein betagamma-subunits (1998), J. Biol. Chem., 273, 7441-7447.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| betagamma-subunit of a photoreceptor G protein | directly activates, 2.5fold | Enteroctopus dofleini | |
| spermine | at low concentrations: up to 20% activation, inhibits at higher concentrations | Enteroctopus dofleini |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ork gene encoding RK is cloned and sequenced | Enteroctopus dofleini |
General Stability
| General Stability | Organism |
|---|---|
| ethylene glycol is used as stabilizer | Enteroctopus dofleini |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dextran sulfate | strong | Enteroctopus dofleini | |
| heparin | potent inhibitor | Enteroctopus dofleini | |
| mastoparan | potent inhibitor | Enteroctopus dofleini | |
| polyanion | e.g. heparin, dextran sulfate, polyglutamic acid | Enteroctopus dofleini | |
| polycation | e.g. polyamines or polylysine, potent inhibitor | Enteroctopus dofleini | |
| Polyglutamic acid | weak | Enteroctopus dofleini | |
| polylysine | potent inhibitor | Enteroctopus dofleini | |
| spermidine | potent inhibitor | Enteroctopus dofleini | |
| spermine | at higher concentrations, activates at low concentrations | Enteroctopus dofleini | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | photoreceptor microvillar membranes, in both the soluble and membrane fractions | Enteroctopus dofleini | 16020 | - |
| soluble | in both the soluble and membrane fractions | Enteroctopus dofleini | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Enteroctopus dofleini | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure | Enteroctopus dofleini |
| 80000 | - |
x * 80000, SDS-PAGE, predicted from the ork gene sequence | Enteroctopus dofleini |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Enteroctopus dofleini | - |
ADP + phosphorhodopsin | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethylene glycol | used as stabilizer | Enteroctopus dofleini |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enteroctopus dofleini | O97020 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | not isoprenylated | Enteroctopus dofleini |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 17.2fold | Enteroctopus dofleini |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Enteroctopus dofleini | - |
| additional information | not in brain, optic lobe, testis, liver, muscle, salivary gland, skin | Enteroctopus dofleini | - |
| retina | photoreceptor microvillar membranes | Enteroctopus dofleini | - |
| retina | retina-specific expression | Enteroctopus dofleini | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.046 | - |
pH 7.4, 15°C | Enteroctopus dofleini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | substrates: octopus rhodopsin, rhodopsin-containing phospholipid vesicles | Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | light-dependent phosphorylation | Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE, predicted from the ork gene sequence | Enteroctopus dofleini |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
assay at | Enteroctopus dofleini |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Enteroctopus dofleini |
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