Activating Compound | Comment | Organism | Structure |
---|---|---|---|
betagamma-subunit of a photoreceptor G protein | directly activates, 2.5fold | Enteroctopus dofleini | |
spermine | at low concentrations: up to 20% activation, inhibits at higher concentrations | Enteroctopus dofleini |
Cloned (Comment) | Organism |
---|---|
ork gene encoding RK is cloned and sequenced | Enteroctopus dofleini |
General Stability | Organism |
---|---|
ethylene glycol is used as stabilizer | Enteroctopus dofleini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dextran sulfate | strong | Enteroctopus dofleini | |
heparin | potent inhibitor | Enteroctopus dofleini | |
mastoparan | potent inhibitor | Enteroctopus dofleini | |
polyanion | e.g. heparin, dextran sulfate, polyglutamic acid | Enteroctopus dofleini | |
polycation | e.g. polyamines or polylysine, potent inhibitor | Enteroctopus dofleini | |
Polyglutamic acid | weak | Enteroctopus dofleini | |
polylysine | potent inhibitor | Enteroctopus dofleini | |
spermidine | potent inhibitor | Enteroctopus dofleini | |
spermine | at higher concentrations, activates at low concentrations | Enteroctopus dofleini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | photoreceptor microvillar membranes, in both the soluble and membrane fractions | Enteroctopus dofleini | 16020 | - |
soluble | in both the soluble and membrane fractions | Enteroctopus dofleini | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Enteroctopus dofleini |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure | Enteroctopus dofleini |
80000 | - |
x * 80000, SDS-PAGE, predicted from the ork gene sequence | Enteroctopus dofleini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Enteroctopus dofleini | - |
ADP + phosphorhodopsin | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | used as stabilizer | Enteroctopus dofleini |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enteroctopus dofleini | O97020 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | not isoprenylated | Enteroctopus dofleini |
Purification (Comment) | Organism |
---|---|
17.2fold | Enteroctopus dofleini |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | retina | Enteroctopus dofleini | - |
additional information | not in brain, optic lobe, testis, liver, muscle, salivary gland, skin | Enteroctopus dofleini | - |
retina | photoreceptor microvillar membranes | Enteroctopus dofleini | - |
retina | retina-specific expression | Enteroctopus dofleini | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.046 | - |
pH 7.4, 15°C | Enteroctopus dofleini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | - |
Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | substrates: octopus rhodopsin, rhodopsin-containing phospholipid vesicles | Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | light-dependent phosphorylation | Enteroctopus dofleini | ADP + phosphorhodopsin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE, predicted from the ork gene sequence | Enteroctopus dofleini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Enteroctopus dofleini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Enteroctopus dofleini |