Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.14 extracted from

  • Bruel, C.; Cha, K.; Reeves, P.J.; Getmanova, E.; Khorana, H.G.
    Rhodopsin kinase: Expression in mammalian cells and a two-step purification (2000), Proc. Natl. Acad. Sci. USA, 97, 3004-3009.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.14

Cloned(Commentary)

Cloned (Comment) Organism
RK gene is cloned and expressed in Pichia pastoris GS115, COS-1 cells and HEK-293 stable cell line, best in COS-1 cells with correct posttranslational modifications Bos taurus

General Stability

General Stability Organism
glycerol does not protect against inactivation during purification Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme is autophosphorylated Bos taurus
phosphoprotein recombinant RK expressed in COS-1 cells is mainly diphosphorylated Bos taurus
side-chain modification enzyme is isoprenylated, recombinant RK expressed in COS-1 cells is mainly farnesylated Bos taurus

Purification (Commentary)

Purification (Comment) Organism
recombinant RK with N-terminal hexahistidine tag, expressed in Pichia pastoris GS115, COS-1 cells: 210-222fold, and HEK-293 cell line Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
eye retina Bos taurus
-
retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 pH 7.4, 30°C, recombinant RK expressed in COS-1 cells, purified in absence or presence of ATP Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 His-tagged recombinant RK expressed in COS-1 cells Bos taurus
6.67
-
 RK from rod outer segments Bos taurus
7.4
-
 assay at Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.67
-
 RK from rod outer segments: 60% of activity is lost with one pH unit change, recombinant RK expressed in COS-1 cells shows broader activity profile Bos taurus