Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Synthetic peptide | corresponding to sequences within opsin loops 3-4 and 5-6 and the C-terminus, bleached rhodopsin as substrate | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study of autophosphorylation, Km values for synthetic peptide substrates: 5 mM or greater, pH 7.4 | Bos taurus | |
0.00062 | - |
rhodopsin | pH 7.4, fully phosphorylated enzyme | Bos taurus | |
0.00064 | - |
rhodopsin | pH 7.4, minimally phosphorylated enzyme | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | rod membranes | Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
1 * 62000, SDS-PAGE | Bos taurus |
62000 | - |
1 * 62000, unphosphorylated kinase, SDS-PAGE | Bos taurus |
64000 | - |
1 * 64000, autophosphorylated kinase, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Bos taurus | major regulatory mechanism for the control of photorhodopsin transduction pathway | ADP + phosphorhodopsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation of serine residues, uneffected by the presence of bleached rhodopsin, results in a transition of the molecular mass to 64 kDa, not a major regulatory mechanism for control of kinase activity | Bos taurus |
phosphoprotein | enzyme is autophosphorylated in the absence of rhodopsin | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + rhodopsin = ADP + phosphorhodopsin | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | retina | Bos taurus | - |
eye | dark-adapted | Bos taurus | - |
retina | rod cell outer segment | Bos taurus | - |
retina | from dark-adapted eyes | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
pH 7.4 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + peptide | corresponding to the C-terminus and loop 5-6 of opsin, poor substrates, phosphorylates serine and threonine residues in each peptide | Bos taurus | ADP + phosphopeptide | - |
? | |
ATP + rhodopsin | catalyzes multisite phosphorylation of purified rhodopsin in phospholipid vesicles | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | light-dependent phosphorylation | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | major regulatory mechanism for the control of photorhodopsin transduction pathway | Bos taurus | ADP + phosphorhodopsin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Bos taurus |
monomer | 1 * 62000, unphosphorylated kinase, SDS-PAGE | Bos taurus |
monomer | 1 * 64000, autophosphorylated kinase, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |