Cloned (Comment) | Organism |
---|---|
- |
Bos taurus |
cDNA encoding enzyme is cloned and expressed in COS-7 cells, sequence of the 561 amino acids protein | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | rod membranes | Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure | Bos taurus |
62900 | - |
x * 62900, calculated from the amino acid sequence | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Bos taurus | light-dependent deactivation of rhodopsin involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase | ADP + phosphorylated rhodopsin | - |
? | |
ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | Bos taurus | light-dependent initiating of deactivation of rhodopsin | ADP + phosphorhodopsin | - |
? | |
additional information | Bos taurus | RK phosphorylates other color opsins in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Bos taurus | P28327 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | - |
Bos taurus |
additional information | the primary structure of enzyme suggests several posttranslational modifications, e.g. myristoylation, phosphorylation, isoprenylation sites | Bos taurus |
phosphoprotein | enzyme is autophosphorylated | Bos taurus |
Purification (Comment) | Organism |
---|---|
1000fold | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | retina | Bos taurus | - |
pineal gland | contains RK mRNA | Bos taurus | - |
retina | rod cell outer segment | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | light-dependent deactivation of rhodopsin involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase | Bos taurus | ADP + phosphorylated rhodopsin | - |
? | |
ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | domain structure, catalytic domain of 270 amino acids in the center of the sequence | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | highly specific for rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | light-dependent phosphorylation | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | phosphorylates serine and threonine residues in the carboxy-terminal region of opsin peptide | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | light-dependent initiating of deactivation of rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
additional information | RK phosphorylates other color opsins in vivo | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Bos taurus |
? | x * 62900, calculated from the amino acid sequence | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
rhodopsin kinase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |