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Literature summary for 2.7.11.13 extracted from
- Substrate recognition mechanism of atypical protein kinase Cs revealed by the structure of PKCiota in complex with a substrate peptide from Par-3 (2012), Structure, 20, 791-801.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 cells as a His-tagged fusion protein using the baculovirus system | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of PKCioata is determined at 2.4 A. The structure of the PKCiota/Par-3 peptide complex reveals that the kinase domain adopts an active conformation even though Thr402 in the activation loop is not phosphorylated. The Par-3 peptide binds to the groove between the N- and C-lobes of the kinase via combined hydrophobic and charge-charge interactions | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q62074 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Par-3 | - |
Mus musculus | ADP + phosphorylated Par-3 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKCiota | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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