Any feedback?
Literature summary for 2.7.11.12 extracted from
- Phosphorylation of NF-kappaB proteins by cyclic GMP-dependent kinase. A noncanonical pathway to NF-kappaB activation (2003), Eur. J. Biochem., 270, 2174-2185.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination of isozyme PKGIalpha, functional co-expression of His-tagged enzyme in 293T cells with human NF-kappaB p49 and p50, NF-kappaB activates PKG, which increases 5fold transactivation activity of p65 from consensus sites but not from a nonconsensus NF-kappaB reporter | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Rp-8-pCPT-cGMPS | - |
Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
isozyme PKGIalpha | - |
| Mus musculus | - |
BALB/c mice, isozyme PKGIalpha | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | native purified from lung or recombinant | Bos taurus | - |
| kidney | - |
Mus musculus | - |
| lung | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + EKRKRTYETF | p65-derived synthetic peptide substrate | Mus musculus | ADP + phosphorylated EKRKRTYETF | - |
? | |
| ATP + Kemptide | i.e. LRRASLG | Mus musculus | ADP + phosphorylated Kemptide | i.e. LRRA-phosphoserine-LG | ? | |
| ATP + Kemptide | i.e. LRRASLG | Bos taurus | ADP + phosphorylated Kemptide | i.e. LRRA-phosphoserine-LG | ? | |
| ATP + MQLRRPSDRE | p65-derived synthetic peptide substrate | Mus musculus | ADP + phosphorylated MQLRRPSDRE | - |
? | |
| ATP + NF-kappaB | recombinant human NF-kappaB p49 and p50 expressed in 293T cells | Mus musculus | ADP + phosphorylated NF-kappaB | - |
? | |
| ATP + NF-kappaB | recombinant human NF-kappaB p49 and p50 expressed in 293T cells | Bos taurus | ADP + phosphorylated NF-kappaB | - |
? | |
| ATP + p65 | activity with recombinant wild-type p65 and mutants T305A and S276A | Mus musculus | ADP + phosphorylated p65 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclic GMP-dependent kinase | - |
Mus musculus |
| cyclic GMP-dependent kinase | - |
Bos taurus |
| PKG | - |
Mus musculus |
| PKG | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Mus musculus |
| 22 | - |
assay at room temperature | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
assay at, substrate Kemptide | Mus musculus |
| 6.9 | - |
assay at, substrate Kemptide | Bos taurus |
| 7.5 | - |
assay at, substrate NF-kappaB | Mus musculus |
| 7.5 | - |
assay at, substrate NF-kappaB | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
| ATP | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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